Simple Patterning Based On Microcontact Printing For Three-Dimensional Cell Spheroid Manipulation

Three-dimensional(3D)cell culture model has become an important culture model in vitro due to its ability to mimic the main pathophysiological characteristics of tumor tissue structure,oxygen concentration,and nutrient gradient.The development of a simple and controllable 3D cell spheroid manipulation and analysis platform is of great scientific significance for advancing the process of cancer therapy and drug discovery.In this study,an easy and a convenient patterning method was developed by using microcontact printing technology,which was used to construct a microarray chip platform,and further research based on this platform for application of 3D tumor spheroid preparation and anti-tumor analysis was conducted.The main research results are as follows:(1)Based on the microcontact printing technology,a simple and rapid patterning novel method without any pre-modification steps and cytotoxic reagents,which was developed for the formation of uniform microarrays.Repeated experiments and quantitative analyses of the prepared microarray patterns demonstrated that the method could achieve reproductive preparation of regular shaped and uniform sized patterning microarrays.(2)The 3D tumor preparation based on the microarray chip platform has been processed,including the uniformly localize and culture of cells,stablely prepare of 3D tumor spheroid and corresponding microarray.It combined with the quantitative analyses and confirmed the applicability of the patterning method and the fabricated chip platform for size-uniform 3D cell spheroid manipulation.(3)The anti-cancer drug trails and apoptosis monitoring research of3 D tumor spheroid on the patterning microarray platform were achieved.And the applied prospect of the platform for 3D tumor drug analysis was confirmed based on the patterning method.In summary,this study develops a simple and convenient patterning method for the 3D tumor spheroid manipulation and analysis application,which can provide a novel methodology for effectiveness and safety evaluation of drugs in the field of drug research and tumor treatment in the future.