The Study Of Adipose-derived Stem Cells Regulating The Secretion Of Different T Lymphocytes Subsets Cytokines

Objectives: Adipose-derived stems cells(ADSCs)are a group of mature stem cells with the capacity of multi-potency,self-regeneration and immunomodulation.ADSCs are easily gained and wildly accepted than bone marrow mesenchymal stem cells(BMSCs).The immunomodulation ability of ADSCs is crucial for homeostasis.ADSCs intervene with the inner immune system to realize immune-regulation.T lymphocytes are the important member of immune system.And cytokines are the main effectors of T lymphocytes.It is crucial to establish the co-culture system to investigate the mechanism of ADSCs immunomodulation on T lymphocytes subsets.Methods: 1.Culture and identification of ADSCs.We use the standard protocol to culture ADSCs.And flow cytometry can detect the surface antigen.Bone induction,adipose induction and cartilage induction are utilized for testing multi-potency.2.Isolation,culture and detection of CD4+ and CD8+ T lymphocytes.3.The construction of ADSCs and T lymphocytes co-culture system.4.The Bioplex analysis of cell supernatant cytokines.Results: 1.The surface markers are according with the accepted ADSCs markers and the ability of multi-potency is fully tested.2.PHA activates CD4+ T lymphocytes to produce Th1 and Th2 cytokines.In the co-culture system of CD4+T lymphocytes and ADSCs,the level of Th1 cytokine is decreased and the level of Th2 but IL-4 cytokine is increased.3.PHA activates CD8+ T lymphocytes to produce Tc1 and Tc2 cytokines.In the co-culture system of CD8+ T lymphocytes and ADSCs,the level of Tc1 cytokine is decreased and the level of Tc2 but IL-4 cytokine is increased.Conclusion: 1.PHA can activate T lymphocytes.2.IFN-γ licensed ADSCs can inhibit Th1 and Tc1 cytokines and promote Th2 and Tc2 cytokines but IL-4.