Research On The Preparation Of Bilberry Anthocyanins Liposomes And The Antioxidant Effect On Cells

Bilberry anthocyanins are widely used bioactive substances that show good effects in scavenging free radicals,antioxidant and anti-inflammatory activities,but their poor stability and susceptibility to degradation largely affects their bioavailability.Liposomes offer advantages over conventional formulations to improve the biostability,absorption and bioavailability of packaging materials.In this project,bilberry anthocyanins were used as the object,and liposomes were used as carriers to optimize the preparation process,evaluate the stability,and affect the morphology,activity,and antioxidant activity of human colon cancer cells Caco-2cells.investigated.The main findings of this article are as follows:1.The anthocyanin liposomes were prepared by the thin film dispersion method,and the encapsulation rate and particle size range of the anthocyanin liposomes were examined using response surface optimization based on a single-factor test;the microstructure of the anthocyanin liposomes was observed using transmission electron microscopy.The results showed that the encapsulation rate of anthocyanin liposomes was 71.21%and the particle size was 213.2±13.4 nm.The results of transmission electron microscopy also showed that the anthocyanin liposomes had a smooth vesicle-like structure.2.To assess the stability of liposomes,it was found that the liposome leakage rate and particle size values gradually increased with storage time,and the pH did not change significantly within 15 d;The simulated gastrointestinal fluid results showed that the liposomes slowly released the encapsulated anthocyanins during the digestion time,and the leakage rate tended to increase significantly after 1.5 h.The liposome leakage rate was relatively low and had some stability.In an in vitro antioxidant assay,the clearance of DPPH by anthocyanin liposomes reached 84.09%at a concentration of 0.05 mg/mL and the clearance of ABTS by anthocyanin liposomes reached 86.09%at its concentration of 0.05 mg/mL.3.The results of cell viability test and microstructure observation showed that anthocyanin liposomes did not significantly change the cell structure within the experimental concentration range.Cell absorption experiments showed that cells could increase the absorption rate in a short time,and the average fluorescence intensity was the highest at 6 h.4.A model of cell damage by hydrogen peroxide was established.When the concentration of H₂O₂reached 200μmol/L,the cell activity was 50.21%under the corresponding conditions.Anthocyanin and anthocyanin liposomes can enhance the total antioxidant capacity of oxidatively damaged cells.Compared with the damaged group,the antioxidant capacity of the treatment group was improved,and the content of T-AOC in the 0.20 mg/mL group was increased by 3.42 times,8.89 times;anthocyanin and anthocyanin liposomes can reduce the content of reactive oxygen species in damaged cells,and the content of malondialdehyde in damaged cells also decreased.Compared with the damage group,the MDA content of the 0.20 mg/mL group decreased by 43.44%and 53.66%,respectively;the activity of endogenous antioxidant enzymes in cells was detected.After treatment with different concentrations of anthocyanin and its liposomes,the activities of SOD and CAT enzymes in oxidatively damaged cells gradually increased.The antioxidant capacity of cells is enhanced.