| Tcf19(transcription factor 19)is an important transcription factor regulating key the genes involved in the cell cycle and apoptotic pathways,and is required for the growth and survival of pancreatic INS-1 cells.Moreover,it binds the promoter of the tumor suppressor gene FOXO1 and suppresses its expression to promote the G1/S phase transition of cancer cells,playing an oncogenic role in a variety of cancers,such as liver cancer,non-small cell lung cancer,colorectal cancer,etc.Overexpression of Tcf19 significantly increases the expression of inflammatory and DNA damage response(DDR)genes.Studies have also found that the Tcf19 gene is upregulated in pro/pre B cells in early human B cell development,and is highly expressed in germinal centers(GCs).Our previous RNA-Seq data also showed a high expression of Tcf19 in GC.These previous studies suggest that Tcf19 is likely to be involved in the development and immune response,especially playing an important regulatory role in the early development of B cells and the humoral immune response.However,few studies on the Tcf19 gene in immune cell development and function have been reported.In order to study the possible regulatory role of Tcf19 in immune cell development and function,we first constructed C57BL/6 Tcf19 knockout mice by CRISPR/Cas9 technology and microinjection,and verified Tcf19 knockout at the DNA and protein levels.Then,and changes of T cells,B cells and their subsets in Tcf19 knockout mice were analyzed by flow cytometry.The basal levels of antibodies in the serum of the mice were measured by ELISA.Littermate wild-type and knockout mice were immunostimulated using thymus-dependent antigen 15% sheep erythrocytes to explore their effects during germinal center formation and antibody production.Our data showed that in contrast to littermate wild-type mice,the appearance of the homozygous mice was not significantly different.The flow cytometry results showed a significant decrease in the pre-pro B cell population in the bone marrow of the knockout mice.The remaining subgroups were not significantly different.There were also no significant differences in the various cell subsets on T cell development stages in thymus.In spleen and lymph nodes,the proportion and cell number of immune cells such as T cells,B cells and NK cells were also not significantly different between wild-type and knockout mice.No significant effects were observed on germinal center formation and Ig G1 antibody generation in Tcf19-/-mice upon thymus-dependent antigen stimulation.These results suggest that the Tcf19 gene may be involved in the early development of B cells. |
