Biological Function Of Three Phenolic Compounds In Minnan Pickled Radish

Minnan pickled radish was produced by traditional methods and considered to be the traditional specialty food in Minnan area of China.Via the long-time pickling,giving radish with unique flavor and taste,in addition promoting intestinal peristalsis,clearing heat and cooling,regulating blood lipids and other food-therapeutic effects.However,there is no clear report to provide a scientific basis for its efficacy.Therefore,this thesis studied the biological function of the phenolic compounds isolated from the Minnan pickled radish,in order to confirm the biological function of Minnan pickled radish,and provide a theoretical basis and scientific basis for the foodtherapeutic effects of Minnan pickled radish.In this study,the biological functions of three phenolic compounds were systematically evaluated by in vitro models,cell model and animal model,and 16s rDNA microbial diversity sequencing was performed to evaluate the regulating effect of three phenolic compounds on the gut microbiota of mice.The main contents and results of this thesis are as follows:(1)Cell viability was determined by MTT assay and it was found that three phenolic compounds could be considered as no cytotoxicity within a certain concentration range.The antioxidant activity of three phenolic compounds were detected by in vitro antioxidant methods,and it was found that DHAP had the highest antioxidant activity among the three phenolic compounds,followed by 4-HPEA and HBA.Via the in vitro bacteriostatic assay found that DHAP and HBA have good antibacterial activity against Escherichia coli,Bacillus subtilis and Candida albicans,while 4-HPEA had no significant effect on the growth of three bacteria.Three phenolic compounds have potential interventions in gout disorders and Alzheimer’s syndrome through in vitro key enzyme inhibitor models.(2)The potential hypolipidemic function of compounds was evaluated by the in vitro nonalcohol fatty liver disease(NAFLD)cell model,it was found that three phenolic compounds have the potential inhibition of intracellular lipid droplets.Compared to the triglyceride(TG)content of model group FFA,DHAP,4-HPEA and HBA had down-regulated by 44.95%,35.32%,and 62.38%,respectively.DHAP did not down-regulate intracellular total cholesterol(TC)content,while 4-HPEA and HBA were down-regulated by 32.93%and 56.88%,respectively.(3)The potential hypolipidemic activity of three phenolic compounds were evaluated by the nutritional obesity mice model.Compared with the positive group SV,DHAP,4-HPEA and HBA have down-regulated by 63.71%,42.35%and 40.90%respectively on the serum TG contents,down-regulated by 38.72%,52.25%and 44.30%respectively on the serum LDL-C contents,upregulated the content of HDL-C,but no significant effect on TC content.The sequencing results of gut microbiota showed that under the intervention of compounds,the structure of the gut microbiota of mice were changed,the abundance,stability and diversity of the gut microbiota of mice were affected,and the gut microbiota disorder caused by high-fat diet were repaired in a certain degree.In summary,through the study of the biological functions of three phenolic compounds such as DHAP,4-HPEA and HBA,it is indicated that the Minnan aged radish may be prevented or assisted conditioning for gout,Alzheimer’s disease,nonalcoholic fatty liver and obesity.And the treatment of three phenolic compounds can adjust the structure of gut microbiota in the experimental dose condition of this study,and provide reference for the development of Minnan aged radish as functional food.