OSA-starch-fatty Acid Research On Preparation,Structure Characterization And Controlled Release Characteristics

Starch has the characteristics of safety,non-toxicity,biodegradability,and good biocompatibility,which can become the carrier of functional factors.However,it has the disadvantages of low loading capacity and easy to be destroyed and degraded by human gastrointestinal environment.Therefore,the core starting point of this paper is to design the starch carrier scientifically and reasonably,so that it can stably transport functional factors to the colon,so as to improve the bioavailability of functional factors.In this paper,based on improving the bioavailability of hydrophobic functional factors represented by curcumin,starch molecules were modified by molecular design to improve the loading performance of functional factors,and amphiphilic anti-digestive nanostarch with colon-targeted controlled releasc was designed.Using high amylose corn starch as raw material,OSA-high amylose corn starch-fatty acid granules were prepared by octenyl succinic anhydride(OSA)esterification combined with fatty acid compound.The optimal preparation process was obtained by response surface experiment,and then the structure and controlled release characteristics of OSA-high amylose corn starch-fatty acid granules were explored.The main research contents are as follows:Fatty acids-high amylose maize starch composites,was prepared with high amylose maize starch as the research object,is digestion resistant modifier with fatty acid,fatty acid compound temperature(20 ℃、25 ℃、30 ℃、35 ℃、40 ℃、45℃、50℃),chain length of fatty acid(C10、C12、C14、C16、C18)fatty acid content(5%、10%、15%、20%、25%)of high amylose maize starch-fatty acids compounds Rapid-Digesting Starch(RDS),Slow-Digesting Starch(SDS)and Resistant Starch(RS)content,the influence of the in vitro digestibility of digestion performance,thus clear the relationship of "complex conditions-fatty acids compounds-digestion performance".Results show that when the reaction temperature is 20℃,and fatty acid content of 20%,carbon chain length of 12,fatty acids-high amylose maize starch digestion resistance,the RS+SDS composition is the highest.According to Box-Behnken central composite design principle,on the basis of single factor experiment,selected OSA content(1%、2%、3%),fatty acid content(15%、20%、25%),length of carbon chain fatty acid(C10、C12、C14)with anti enzyme solutions group points(RS+SDS)and load as the response value,through the response surface method for OSA-high amylose maize starch-fatty acids compounds build optimization process,explore the OSA-high amylose maize starch-fatty acids compounds the key factors in the process of building.By response surface analysis,symplectic alkenyl succinic anhydride-high amylose maize starch particles-fatty acids fatty acid content is the best preparation conditions of 18.25%fatty acid,length of carbon chain fatty acid is 12.57,OSA added amount of 2.58%。under the best conditions for the sheen of alkenyl succinic anhydride-high amylose maize starch particles-fatty acids of(SDS+RS)content of the theoretical value is 60.72%,Factual verification value is 59.36%,curcumin loading capacity of the theoretical value is 6.28 mg/g,the actual test value is 6.40 mg/g.Fitting model and the actual test with consistency.The OSA-high amylose maize starch-fatty acid granules prepared after response surface optimization were used as the research object to explore its structure and characteristics,and analyze the crystal morphology of starch,the change of functional group characteristic peaks of starch granules,and the thermal stability of starch.The results showed that the hydroxyl group of starch was replaced after the introduction of OSA group,and the esterification reaction between OSA and starch was carried out in the amorphous region of starch,resulting in the loose crystal structure of starch and the low gelatinization temperature.Fatty acids and high amylose corn starch will form V-type crystallization,fatty acids will make starch molecular association degree is large,molecular arrangement is closely,thermal stability is enhanced;the cumulative release rate of curcumin in the stomach and small intestine was 17.66%,indicating that OSA-high amylose corn starch-fatty acid can successfully transport curcumin to the colon environment and improve its bioavailability.To provide demonstration and reference for the development and application of functional factors with poor solubility or oral bioavailability.