The Characteristics Of ADH-related Active Component In Tea Saponin And Its Application In Nanoemulsion

Magnetic nanoparticles(MNPs)is one of the research hotspots in recent years.Using Magnetic nanoparticles to immobilize enzymes can improve the stability and reusability of enzymes,and can also be used for screening active components of traditional natural products.Tea saponin has many monomers and similar structures,so it’s difficult to screen active components of tea saponin.Alcohol dehydrogenase(ADH)is a key enzyme in human Alcohol metabolism.It has been reported that tea saponin can inhibit the activity of Alcohol dehydrogenase.It is an efficient and simple method to screen ADH-related active components from crude tea saponin solution by immobilized enzyme technology.Therefore,based on the previous extraction method of our laboratory,this study mainly investigated the physical and chemical properties and antialcoholic effect of the ADH-related active components.The specific contents and conclusions are as follows:The physical and chemical properties of ADH-related active components(TSI)has been studied.The Critical Micelle Concentration of TSI is 0.3%,which shows that TSI can significantly reduce the surface tension of water,the Contact Angle is less than90° and increases with decreasing of concentration of TSI,showing good wettability.The foamability and foam stability of TSI increase with the increase of its concentration,and the best temperature of foamability is 50℃,it also has a good ability to resist hard water and acid and alkali.TSI has good emulsifying performance,and the solubilizing ability of benzene is 7 m L/g.When used alone,the decontamination ability of TSI is not strong,but when it was combined with other surfactants,the effect is obvious.With wetting,foaming,foam stability,emulsifying,solubilizing,decontamination and other properties,TSI is an excellent surfactant.The antialcoholic effect of TSI and its effect on liver and stomach was explored.By intragastric administration of 56° Red Star Erguotou to mice,the optimal level of intoxication was 0.14 m L/10 g.bw,and TSI group with high dose could significantly prolong ebriety time and shorten sober time.Studies on the effects of TSI on the liver and stomach of intoxicated mice showed that the medium and high dose groups of TSI could significantly reduce the liver index and blood transaminase content of mice,improve the activities of SOD and GSH-Px,reduce the content of MDA,enhance the antioxidant level in mice,and reduce the damage of oxygen free radicals to mice.In addition,the activities of ADH and ALDH in liver cells and stomach cells were increased of TSI medium and high dose groups,and ethanol metabolism was promoted.Fluorescence quantitative PCR was used to analyze the gene expression during alcohol metabolism.It was found that the expression levels of pro-inflammatory factors of TNF-α and IL-6 were significantly down-regulated in TSI high-dose group,and the expression levels of ethanol metabolism enzymes ADH1 and ALDH2 genes were significantly increased,which helped to explain the regulatory and protective effects of TSI on ethanol-induced gastric cell and liver cell injury.Garlic oil nanoemulsion(GOE)was prepared and its antibacterial mechanism was preliminarily explained.GOE was prepared by ultrasonic emulsification method.It was found that the optimal preparation conditions of GOE were as follows: the content of tea saponin is 1.5%,the content of garlic oil is 8%,ultrasonic power is 225 W and ultrasonic time is 10 min.GOE has obvious inhibitory effect on Staphylococcus aureus and Listeria monocytogenes,but not on Escherichia coli.According to the bacteriostatic curve,when GOE concentration is 2MIC and MIC,the bacteriostatic effect is strong;when GOE concentration is 1/2MIC,the bacteriostatic effect is weak,but it still has a certain inhibitory effect.By scanning electron microscopy(SEM),AKP enzyme activity assay,conductivity measurement and leakage of nucleic acids and proteins,the bacteriostatic effect of GOE is due to destroy the cell wall of bacteria,leading to make the bacteria distort and out of shape,cause AKP enzyme which between the cell wall and cell membrane leak,and then damage the cell membrane,leading to ion and electrolyte materials of cell leak and the conductivity of cell increase.Leakage of DNA and proteins also affects the physiological activity of the cell,resulting in inhibition of bacterial growth or death.