Regulation Of Functional Groups And Properties Of Triple-shell Hollow Mesoporous Organosilica Nanoparticles For Tumor Photothermal Therapy

Multiple-shell hollow mesoporous organosilica nanoparticles（HMONs）have high specific surface area,large pore volume,tunable framework components and multiple independent chambers,which is divided by the multiple shells,broadening the application prospect of HMONs in the drug delivery,biological imaging,and multimodal therapy.However,most of the reported multi-shell HMONs have complex construction methods,uncontrollable number of shell layers and single functional group components and functions,which limit their further applications in nanomedicine.In this work,multi-component and multi-shell HMONs construction method was established,and a variety of organic group-doped three-shell HMONs nanoparticles were successfully constructed.Furthermore,the triple-shell HMONs nanoparticles were used to support copper sulfide（Cu S）quantum dots to study their photothermal therapy effect for cancer.The main research contents are as follows:1.Triple-shell hollow mesoporous organosilica nanoparticles hybridized with ethane-,thioether-and benzene-groups were prepared by self-transformation method.solid mesoporous organosilica nanoparticles with different condensation degree were synthesized by adding the mixture of organosilane precursor step by step in the directing of cetyltrimethylammonium bromide（CTAB）,Then,the solid mesoporous organosilica nanoparticles can self-transform to triple-shell HMONS（ht HMONs）via hydrothermal treatment.The prepared ht HMONs possess fascinating properties,such as hybridized framework with various functional groups,triple-shell hollow structure,uniform size（320 nm）,high specific surface area(458 m²g^-1)and mesoporous channels（4.20 nm）.By controlling different reaction parameters（such as the amount of surfactant and the volume ratio of water/ethanol）,ht HMONs with different diameter（190-330 nm）have been prepared.In comparison to the standard hard template and soft template methods,this technology eliminates the need to remove the hard template,simplifies the operation processes,ensures consistent morphology and size,and provides good dispersion.This study introduces a novel way for creating multi-component,multi-shell hollow structural materials.2.Triple-shelled hollow mesoporous organosilica/copper sulfide composite nanoparticles（t HMONs@Cu S）were prepared for tumor photothermal treatment.The results showed that t HMONs@Cu S possesses a multi-shell-separated cavity structure,rough shell surface,consistent size（345 nm）,and mesoporous channels as-prepared（3.8 nm）.Photothermal performance tests showed that t HMONs@Cu S had good photothermal conversion efficiency（33.23%）and photothermal stability.In addition,In vitro cell tests revealed that t HMONs@Cu S co-incubated with cells increased cell viability by more than 80%.The tumor cell survival rate was lowered to 40%after laser irradiation with t HMONs@Cu S.The work explores the application potential of multi-shell hollow composite nanomaterials in tumor therapy,and provides a new strategy and method for the future application research of multi-shell hollow nanomaterials.