Transition Metal Phosphide And Molecularly Imprinted Electrochemical Sensors For The Detection And Analysis Of Quinol In Meat

However,some unscrupulous traders add excessive amounts of Olaquindox(OLA)to animal feed to reduce costs and improve feed conversion and leanness.As a new type of lean meat,also known as quinamyl alcohol,fastidious,and beiyuno,it can effectively inhibit the synthesis of bacterial deoxyribonucleic acid(DNA)and has good antibacterial and anticoccidial effects.It is therefore widely used in livestock and aquaculture.Studies have found that quinol is genotoxic,reproductive toxic,teratogenic and mutagenic,and that long-term or heavy use of quinol,which accumulates in animals,poses a potential health risk to humans.Current detection methods have their own advantages,but there are also some drawbacks such as high costs and the accuracy of detection needs to be improved.Therefore,it is necessary to develop a method for the determination of quinol in meat for food safety monitoring and control throughout the meat production process.This paper is based on an electrochemical method for the development of a rapid and sensitive quantitative analytical sensor for the highly sensitive detection of OLA residues in edible meat products.The main thrust of this paper are:1.A simple and rapid electrochemical sensor for the detection of OLA in meat was constructed by combining a hydrothermal method,a carbon coating process and a phosphating treatment to construct a carbon coated hollow iron phosphide(H-FeP-C)modified electrode,and was able to use the electrocatalytic oxidation of OLA by the modified electrode.The morphology and structure of the electrode were characterized by Fourier Transform Infrared Spectrometry(FT-IR),X-ray diffraction(XRD)and X-ray photoelectron spectroscopy(XPS),and the material was successfully synthesized by cyclic voltammetry(CV).The materials were successfully synthesized and found to have good electrochemical properties towards OLA by cyclic voltammetry(CV).The results showed that the linear range of H-FeP-C/GCE for OLA detection was 0.015 μmol/L～29.54 μmol/L with a detection limit of 4.6 nmol/L(S/N=3).Meanwhile,the sensor has good selectivity,reproducibility and stability,and has been successfully used for the detection of beef,pig liver and chicken meat.2.A molecularly imprinted sensor for the rapid detection of quinol was prepared using nano-silver solution and nano-graphene as the electrode modification materials,quinol as the template molecule and o-aminophenol and resorcinol as the composite functional monomers.The experimental conditions such as the molecularly imprinted sensor membrane were optimized,and the reproducibility and anti-interference and stability of the electrode were experimented,which showed good test results with a wide linear range for quinol,with detection limits of 2.6×10-9～1.2×10-8 mol-L-1.The present sensor has good reproducibility and stability with high selectivity,and was used in the OLA of real samples The spiked recoveries were 89.05%～100.86%for the determination.