Study On The Detection Method Of Various Veterinary Drug Residues In Poultry Meat And Processed Meat Products

Poultry meat and processed meat products can provide a variety of nutrients for human growth and development.These products have become an indispensable food on people’s table by reason of the widely favored by the public as one of the most important protein sources.As a widely recognized environmental pollutant,veterinary drugs are applied to nearly 80% animals in the livestock farming industry,and their residues have become a popular social issue.However,the higher fat and protein content,as well as the additives and excipients added during processing to improve the flavor of the product or extend their shelf life,will produce greater interference for detecting multi-veterinary drug residues,and increase the difficulty of the analysis.Therefore,an easy,quick,cheap,effective,rugged,safe,high-selectivity and sensitive pretreatment method coupled with a method that can achieve high-throughput,multi-residue detection of veterinary drugs,and achieve accurate quantification of the target analytes,is in urgent need of establishment.It has significant practical application value for ensuring food quality and protecting consumer health.In this study,a rapid and sensitive technique for determining multiple veterinary drug residues in poultry meat and processed meat products was developed using a modified Qu ECh ERS method combined with ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MSMS).It was successfully applied to detect multiple veterinary residues in marketed poultry meat and processed meat products.The main research contents are as follows:(1)A method for detecting 67 veterinary drugs in poultryA modified Qu ECh ERS method combined with UPLC-MSMS was applied to develop a rapid and sensitive method for determining 67 veterinary drug residues in pigeon and chicken meat.At first,three significant influencing factors were selected from 11 influencing factors of Qu ECh ERS method by Plackett-Burman(P-B)screening experimental design,Then,a three-level,three-factor BBD design was used to optimize the Qu ECh ERS method by response surface methodology.The optimal experimental parameters were determined to achieve accurate quantitative and qualitative analysis of 67 veterinary drugs in pigeon meat samples.The experimental results showed that,the excellent linearity of the calibration curve was shown in the corresponding concentration range with a coefficient of determination greater than 0.99.The relative standard deviations(RSD)were expressed as intra-day and inter-day precision were less than 9.5% and 19.9%,respectively.The modified Qu ECh ERS method further simplifies the pre-treatment steps.The developed method was suitable for the high-throughput qualitative screening and quantitative detection of multiple veterinary drug residues in pigeon and chicken meat,which can provide a basis for routine analytical testing of poultry and its related matrix.(2)A method for detecting 43 antimicrobial drugs in processed meat productsThis study applied a modified Qu ECh ERS method combined with UPLC-MSMS to develop a rapid and sensitive method for determining antimicrobial drugs in bacon and ham.A three-level,three-factor BBD design was used to optimize the Qu ECh ERS method by response surface methodology.The optimal experimental parameters were determined to achieve accurate quantitative and qualitative analysis of 43 antibacterial drugs in bacon and ham.The experimental results showed that,the excellent linearity of the calibration curve was shown in the corresponding concentration range with a coefficient of determination greater than 0.99.The values of decision limit(CCα)and detection capability(CCβ)were in the range of 10.9-31.3μg/kg and 11.8-52.5μg/kg,respectively.Box-Behnken response surface design effectively reduced the number of experiments,reagent consumption,time required,and achieved satisfactory results.It was suitable for the routine analysis of multiple anti-microbial drug residues in processed meat products.(3)Application in the commercial samplesThe established methods were used for screening analysis and quantifying confirmation of veterinary drug residues in 40 commercially available samples(including 6 pigeon samples,6 chicken samples,14 bacon samples and 14 ham samples,)from local supermarkets.The results showed that there were three samples detected three veterinary drugs.Tilmicosin in the chicken sample was 4.4μg/kg,sulfadiazine and moxifloxacin detected in bacon and ham samples at the concentrations were 0.6 and 10.9μg/kg,respectively.