Application Of Polyionic Complex Micelles Based On Carbon Quantum Dots In Tumor Therapy And Dignosis

The diagnosis and treatment of tumors have significant clinical and scientific research implications.Quantitative detection of tumor markers is an important criterion for identifying cancer in the field of clinical medicine,and various emerging anti-tumor treatment strategies have also received attention,becoming a hot spot in anti-tumor research.However,the available donor materials that can be used in the field of tumor diagnosis and treatment are very limited.Among them,polyelectrolyte complex micelles can be used as one of the best elemental materials for drug delivery and sensing detection due to their excellent biocompatibility.Therefore,this paper selects polyelectrolyte complex micelles based on carbon quantum dots as unit materials,and applies them to the detection of biological biomarkers thiol in anti-tumor research and tumor diagnosis.The specific work is as follows:（1）We report a NO-based gas therapy treatment strategy,in which a polyelectrolyte complex nanocarrier（m PA-NP）is formed by electrostatic assembly between amphiphilic anionic polymers and positively charged NO donors with a size distribution of around 110 nm.The nanocarrier can generate NO nanobubbles in the core upon H₂O₂stimulation,which can be used as a nanobomb in anti-tumor research.In vitro experiments demonstrated that m PA-NP nanocarriers could achieve lysosomal escape and possess multi-organelle destruction capabilities when taken up by cells.Moreover,in vivo experiments further confirmed the good tumor-inhibiting effect of the polyion complex micelles.（2）Polyionic complex micelle（mPH-NP）were synthesized using histidine carbon dots（H-CDs）and block copolymers（PEG-b-PAsp）through electrostatic assembly,an average size of approximately 115 nm and used as sensing elements to construct a sensor array for qualitative and quantitative analysis of thiols.Based on the concentration effect of sensing elements and the ion cage trapping effect,the sensing and detection process can be carried out inside the core of the micelles,greatly improving the sensitivity and accuracy of the detection.The multidimensional sensing platform achieved accurate identification of seven thiols in serum with detection concentrations ranging from 0.5μM to 1000μM.Quantitative detection of GSH/Hcy/Cys in multicomponent systems was achieved through support vector machine,with accuracy above 90%.Finally,it can distinguish normal cells from cancer cells based on the different levels of GSH in different types of cells.