| As a clean hydrogen production method,biological hydrogen production mainly includes photo-fermentation biological hydrogen production and dark-fermentation biological hydrogen production.In the context of the rapid development of global renewable energy,biological hydrogen production is considered to be the most potential way of hydrogen production.At present,biological hydrogen production reactors are mainly divided into tubular photo-fermentation biological hydrogen production reactors and plate photofermentation biological hydrogen production reactors,but because of their large volume and long operating cycle,they can not be widely used.Portable photo-fermentation biological hydrogen production reactor can be suitable for various application scenarios in daily life.Aiming at portable photo-fermentation biological hydrogen production reactor,the effects of enzyme load,strain concentration and Fe2+ on the operation of portable photo-fermentation biological hydrogen production reactor were studied.By analyzing the basic parameters in the process of photo-fermentation biological hydrogen production,The process conditions of biomass ardonax were optimized as substrate of photo-fermentation.The conclusion is as follows:(1)Using Auto-CAD,Solidworks and other softwares to design and optimize each unit of the portable photo-fermentation biological hydrogen production reactor,aiming to produce a portable and efficient photo-fermentation biological hydrogen production reactor.Considering the structure of each unit and the connection mode between each unit,the structure size of the reactor was determined according to the hydrogen demand,the material of the reactor was screened according to the unique environmental characteristics required by photo-fermentation biological hydrogen production,and the basic operating conditions of the portable photo-fermentation biological hydrogen production reactor were determined according to the specific environment required by the growth and metabolism of photosynthetic bacteria HAU-M1.At the same time,the matching of thermal insulation and light units can ensure the efficient metabolism of photo-synthetic bacteria HAU-M1 to produce hydrogen.(2)The effects of enzyme load,strain concentration and Fe2+ on the operation of portable photo-fermentation biological hydrogen production reactor were studied.The p H,ORP,reducing sugar concentration,volatile fatty acid content,enzyme activity and other parameters of fermentation liquid in the process of photo-fermentation biological hydrogen production were analyzed to determine the optimal process conditions,and the influence of each parameter was explored by scatter matrix analysis.When the enzyme load was 20 FPU/g,the cumulative hydrogen production was 258.99 m L and the delayed hydrogen production period was 11.19 h,which was the shortest in the same experimental group.When the strain concentration was 2.88 g/L,the cumulative hydrogen production was 260.26 m L,and the hydrogen production delay was 1.85 h,which was the shortest in the same experimental group.When the concentration of Fe2+ was 20mg/L,the cumulative hydrogen production was 342.57 m L and the delay period of hydrogen production was 8.00 h.(3)The content of nitrogen-fixing enzyme and acetic acid dehydrogenase in fermentation liquid was determined by enzyme-related immunosorbent.At 12 h in the early stage of photolytic fermentation,the content of nitrogen-fixing enzyme in the experimental group with an enzyme load of 20 FPU/g(148.48IU/L)was 26.67% higher than that in the group with 50 FPU/g(117.21IU/L).The content of acetic acid dehydrogenase was 8.7% higher at 20 FPU/g(38.86U/L)than at 50 FPU/g(35.73U/L).The nitrogen fixing enzyme content of 2.88 g/L photosynthetic bacteria HAU-M1(98.92IU/L)was 42.23% higher than that of 4.77 g/L(69.55IU/L)at 24 h.The content of acetic acid dehydrogenase in 2.88 g/L(18.16U/L)HAUM1 was 21.23% higher than that in 4.77 g/L(14.98U/L).With the change of Fe2+ concentration,at 24 h,the Fe2+ concentration of 20mg/L group(98.92IU/L)was 36.78% higher than that of the group with the longest delay period of hydrogen production(69.55IU/L).The content of acetic acid dehydrogenase had the greatest difference at 12 h,and the experimental group of 20mg/L Fe2+(39.61U/L)was 40.16%(28.29U/L)higher than that of the experimental group with the longest delay period of hydrogen production. |
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