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Development Of Lateral Flow Immunoassay Test Strip For Quantitative Detection Of Fumonisin B1 Based On Aggregation-Induced Emission

Posted on:2024-05-05Degree:MasterType:Thesis
Country:ChinaCandidate:G XuFull Text:PDF
GTID:2531307100496334Subject:Master of Science in Biology and Medicine (Professional Degree)
Abstract/Summary:PDF Full Text Request
Fumonisin is a common mycotoxin produced mainly by Fusarium and often contaminates corn and other crops.At present,28 kinds of fumarins have been found,including FA1,FA2,FB1,FB2,FB3,FB4,FC1,FC2,etc.Among them,FB1 is the main component and the most toxic component.FB1 can be detected by instrumental analysis method.Although this method has good accuracy and reliability,it requires expensive equipment,complex procedures and skilled technicians,which limits its application in rapid on-site detection of FB1.Lateral flow immunoassay(LFIA)has the advantages of high speed and simplicity,high sensitivity,good specificity and low cost,and plays an important role in rapid field screening in food safety and resource-poor areas.LFIA based on fluorescent microbeads has been widely concerned for its ability to realize rapid and sensitive monitoring of food safety.However,traditional fluorescent microbeads are restricted by the aggregation-caused quenching(ACQ)effect of the loaded fluorophores,thus resulting in low signal intensity and insufficient sensitivity of fluorescent LFIA.Herein,ultrabright AIE fluorescent microbeads(AIEMBs)were successfully prepared by an emulsification method to encapsulate green-emitting fluorophores featured with aggregation-induced emission(AIE)characteristic into polymer nanoparticles.Subsequently,the as-prepared AIEMBs was used as signal probes to develop competitive LFIA(AIE-LFIA)for the rapid and highly sensitive detection of FB1 in real corn samples.Under the optimal experimental conditions,the regression equation of FB1 in corn samples detected by AIE-LFIA established in this study was y=-0.189ln(x)+0.1923,with a dynamic detection range from 0.024 ng/m L to 3.125 ng/m L.The half inhibitory concentration(IC50)and the lowest detection limit(LOD)were 0.20 ng/m L and 0.024 ng/m L,respectively.The average recoveries for the intra-and inter-assays range from 84.8%to 114.0%with coefficients of variation(CV)ranging from 4.05%to 7.82%.In addition,there was no obvious cross-reaction between the method and other common mycotoxins.The above results show that the proposed AIE-LFIA has good selectivity,accuracy and reliability,which provides a new technical support for the rapid and sensitive screening of FB1.Compared with the traditional single-signal LFIA,the dual-signal LFIA integrates the advantages of different detection signals.The dual-signal probe based LFIA has become a research hotspot due to its excellent analytical performance and huge application potential.In addition,the detection results of dual-signal LFIA can be mutually verified,making the results more accurate and reliable.Herein,fluorescence colorimetric dual-signal microbeads(AIE@Au NPs)were prepared by the microemulsion method by doping with hydrophobic gold nanoparticles(OA-Au NPs)and AIEgens.The obtained AIE@Au NPs integrate excellent colorimetric signal of Au NPs and excellent luminescence signal of AIEgens to realize dual-mode colorimetric fluorescence signal output.Given this,the synthesized AIE@Au NPs were then used as dual-signal probes to establish a highly sensitive colorimetric/fluorescence dual-mode LFIA(AIE@Au-LFIA)for the quantitative detection of FB1 in maize samples.Under optimal experimental conditions,the IC50values of FB1 in corn samples by AIE@Au-LFIA method were 0.20 ng/m L and 0.23ng/m L,respectively,based on colorimetric and fluorescence modes.The linear ranges were 0.078~0.625 ng/m L for colorimetric detection and 0.039~2.5 ng/m L for fluorescent detection.The above results show that the sensitivity of the two detection modes of the established AIE@Au-LFIA is similar,but fluorescence mode has a wider detection range.Therefore,the dual-mode AIE@Au-LFIA based on AIE@Au NPs has a good application potential in the field of rapid detection owing to its higher accuracy.
Keywords/Search Tags:Lateral flow immunoassay, Fumonisin B1, Aggregation-induced emission microbeads, Dual-signal microbeads
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