Construction And Drug-controlled Release Performance Of Mussel-inspired Functional Bioglass/fibroin Three-dimensional Composite Fibrous Scaffolds

Copper-doped mesoporous bioactive glasses(Cu-doped mesoporous bioactive glasses,Cu-MBG)with unique excellent properties have been used as stimulus-responsive drug carriers in the drug delivery system.On the other hand,their good bioactivity and biocompatibility are widely used as bone repair materials in the bone tissue engineering.In this paper,Cu-MBG were firstly prepared by sol-gel method,and further mussel-inspired with polydopamine(Polydopamine,PDA)coating to obtain polydopamine doped copper mesoporous bioglasses(Cu-MBG@PDA)nanoparticles.In this regard,Effect of different concentrations of PDA on the structural characteristics and performance of Cu-MBG@PDA nanoparticles were investigated.To select silk fibroin(SF)from a wide range of sources and then prepare three-dimensional composite nanofibrous scaffolds from a mixture of Cu-MBG@PDA and SF by wet electrospinning technique,and to study the effect of the spinning solution ratio on their properties.Finally,VAN was loaded into Cu-MBG@PDA as a model drug to prepare VAN@Cu-MBG@PDA samples,and then wet electrostatic spinning was applied to polymerize SF and VAN@Cu-MBG@PDA were polymerized together using wet electrostatic spinning to make drug-loaded 3D SF/VAN@Cu-MBG@PDA fibrous scaffolds,and the adsorption and in vitro release properties of VAN@Cu-MBG@PDA and SF/VAN@Cu-MBG@PDA on VAN were discussed,and their adsorption and release mechanisms were explored,and the following conclusions were mainly obtained.(1)Preparation and relative property of mussel-inspired Cu-MBG nanoparticlesCu-MBG@PDA nanoparticles were prepared by further PDA coating modification on the surface of Cu-MBG as a substrate,and it was confirmed that PDA had been successfully modified on the surface of Cu-MBG by means of FTIR,SEM,XRD and EDS characterisation;the hydrophilicity,bioactivity and antibacterial properties of the samples were better when the modified PDA modification concentration was 4 mg/m L.The antibacterial mechanism of Cu-MBG@PDA4 nanoparticles was found to be the synergistic antibacterial effect produced by both PDA and copper ions,resulting in the best antibacterial effect of Cu-MBG@PDA nanoparticles in the tested samples.(2)Preparation and properties of mussel-inspired like functional bioglass/SF three-dimensional composite fiber scaffoldThree-dimensional pure SF and SF/Cu-MBG@PDA fiber scaffolds were successfully prepared by wet electrostatic spinning method using Cu-MBG@PDA4nanoparticles as the substrate.The results showed that the porosity,mechanical properties,bioactivity,biodegradation and antibacterial properties of 3D SF/Cu-MBG@PDA were significantly improved with the increase of Cu-MBG@PDA4content compared with the pure 3D SF scaffolds.However,when the mass of Cu-MBG@PDA4 in the SF/Cu-MBG@PDA spinning solution was 3 mg,a series of properties of the prepared SF/3Cu-MBG@PDA4 were superior.(3)Preparation of SF/VAN@Cu-MBG@PDA fiber scaffolds and controlled drug release studiesThe adsorption performance of VAN@Cu-MBG@PDA was investigated.When the p H of VAN solution was 7.4,the initial concentration and adsorption time of VAN drug molecule adsorption were 25 mg/m L and 100 min,respectively,the maximum amount of VAN@Cu-MBG@PDA adsorption was reached,the equilibrium adsorption amount was 14.02 mg/g,the drug loading rate was 8.69%,and the encapsulation rate was 85.38%.The in vitro drug release performance of VAN@Cu-MBG@PDA and SF/VAN@Cu-MBG@PDA was investigated by adjusting the p H(5.5,6.8 and 7.4)and release temperature(30 ℃,37 ℃,40 ℃ and 45 ℃)and validated with a drug sustained release model.The results showed that the drug-loaded VAN@Cu-MBG@PDA and SF/VAN@Cu-MBG@PDA materials showed the drug release profiles were consistent with the Korsmeyer-Peppas model at different p H values and temperatures.The above experimental results show that the 3D SF/VAN@Cu-MBG@PDA nanofiber composite scaffold material has good bioactivity,degradability and antibacterial properties,as well as controlled drug release properties can effectively promote the repair of bone defects,and the material can be used as a bone tissue repair material for the clinical treatment of bone defects in the future.