Fabrication Of Janus Nanomaterials Based On MOFs For Cancer Theranostics

Malignancy has become an urgent global threat,and the high efficiency of chemotherapy makes it an integral part of oncology treatment.Single treatment has great limitations due to the heterogeneity of tumors,synergistic therapy can destroy cancer cells more effectively and thoroughly,which has attracted widespread attention.In this work,two kinds of Janus nanoparticles（JNPs）based on MOFs were synthesized using Prussian blue（PB）and drug-loaded nanoparticals,which synergizes photothermal therapy,chemotherapy and chemodynamic therapy.And then the drug loading rate,drug release performance,photothermal performance and chemical kinetic properties of the composites under different conditions were explored.The specific research works are as follows:（1）The Prussian blue/polyacrylic acid@MIL-100 Janus nanoparticles（PB/PAA@MIL-100 JNPs）were prepared by sol-gel method.The PB NPs on one side of the nanoparticles can provide good photothermal therapeutic effect,the photothermal conversion efficiency can reach to 63.6%and have good photothermal cycle stability.The MIL-100 NPs on the other side are porous with a high specific surface area,it can be used as drug delivery vehicles with a high drug load（0.25 mg DOX）.At the same time,the structure of MIL-100will degrade in the weakly acidic conditions of the tumor environment,the released Fe³⁺will undergo redox reaction with glutathione（GSH）in the tumor environment to generate Fe²⁺,it will react with H₂O₂ to produce reactive oxygen,then the reactive oxygen reacted with methylene blue（MB）.We investigated the chemodynamic therapeutic effect of PB/PAA@MIL-100 JNPs by degradation of MB under different conditions.PB/PAA@MIL-100 JNPs realize the combination of chemotherapy,photothermal therapy and chemodynamic therapy,thereby destroying cancer cells to a greater extent.（2）The same method was used to form PB/PAA NPs with a Janus structure,and then added chloride hexahydrate（Gd Cl₃·6H₂O）to the aqueous solution of PB/PAA Janus structure,the Gd³⁺will hydrolyze on the surface of PAA to generate basic gadolinium hydroxide（Gd（OH）₃）,finally,a layer of mesoporous silica（m Si O₂）was coated to obtain PB/PAA@Gd（OH）₃@m Si O₂JNPs.The loading of synthetic JNPs with doxorubicin（DOX）was 0.55 mg,and the photothermal conversion efficiency reached 59.3%.A combination of photothermal therapy and chemotherapy was realized.At the same time,Gd（OH）₃ will be converted to Gd³⁺under acidic conditions of the tumor,thereby enhancing the effect of magnetic resonance imaging（MRI）.In addition,the drug release behavior of PB/PAA@Gd（OH）₃@m Si O₂ JNPs under the weak acidic conditions and NIR laser irradiation were investigated.