| Hydrogen is not only a clean,low-carbon green energy,but also acts as an antioxidant and messenger molecule in the biomedical field,and has broad application prospects in the prevention and treatment of major diseases.Hydrogen can selectively reduce hydroxyl radicals(·OH)and peroxynitrite(ONOO~-),regulate the oxidative stress marker MDA,and increase the content of SOD(antioxidant enzymes)to enhance the antioxidant effect;The content of pro-inflammatory cytokines and antioxidant enzymes enhances the phagocytic ability of macrophages,so that animal body functions tend to be normal.The current hydrogen treatment strategies are mainly through the following three ways:direct inhalation of hydrogen-oxygen mixture,intravenous injection or drinking hydrogen-rich water.Due to the low solubility of hydrogen in water,it cannot achieve long-term stable storage in a physiological environment;in addition,hydrogen has a low molecular weight and small volume,which is easy to pass through various physiological barriers and diffuse rapidly in tissues.Therefore,traditional hydrogen treatment methods often suffer from hydrogen concentration.Instability,low utilization rate,poor treatment effect and other problems.Aiming at the above problems,this study developed a self-driven electrocatalytic hydrogen therapy system:converting biomechanical energy into electrical energy via a rotating-disc triboelectric nanogenerator(RD-TENG),and in situ electrolysis of water in the biological system to realize hydrogen production.stable release.This strategy will improve the current status of intermittent and unstable hydrogen therapy,and improve the therapeutic effect by maintaining a stable hydrogen concentration locally,and effectively inhibit the proliferation of cervical cancer cells.In this paper,two RD-TENGs with the same structure and different sizes were fabricated by assembling PTFE,aluminum foil and acrylic sheet according to the principles of triboelectric electrification and electrostatic induction.In this study,the maximum open-circuit voltage of RD-TENG was 3 KV and the maximum short-circuit current was 100μA under the condition of 20-80 rpm rotation speed by electrometer and oscilloscope,and the charge transfer amount under this condition was 0.6μC.The above experiments confirm that the RD-TENG system has a stable electrical output,and its output performance is enhanced with the increase of the friction layer contact area and rotational speed.In this study,carbon-based cobalt cathode catalysts and iron-nickel alloy anode catalysts were further prepared by hydrothermal synthesis and pyrolysis,and the annealing temperature was changed to achieve precise control of the catalyst morphology and electronic structure.The prepared carbon-based cobalt cathode catalyst has a scale of200 nm and a HER overpotential of-197 mV under acidic conditions,and an iron-nickel alloy anode catalyst with a scale of 500 nm and an OER overpotential of 325 mV under alkaline conditions.The cathode and anode catalysts were loaded on the interdigital electrode by spin coating to construct a high-performance flexible electrode for water electrolysis that can be used in living organisms.The CCK-8 detection method confirmed that the interdigitated electrode material,carbon-based cobalt hydrogen evolution catalyst and iron-nickel alloy oxygen evolution catalyst have high biological safety.The electrical output provided by RD-TENG promotes the in-situ electrolysis of water to produce hydrogen on the electrode surface,and the released hydrogen can effectively inhibit the proliferation of cervical cancer cells.In summary,this study successfully built a self-driven electrocatalytic hydrogen therapy system to achieve hydrogen inhibition of cervical cancer cell proliferation. |
