| Objective By constructing mouse models with different irradiation doses and irradiation methods,using the prepared BPIFA2 polyclonal antibody,to explore the sensitivity of serum BPIFA2 to radiation and whether it is a biomarker for predicting fatal radiation injury in the early stage of the accident.Methods The recombinant plasmid p GEX-4T-AB1-bpifa2 was constructed by molecular cloning method and transformed into Rosetta(DE3)to induce the expression of the recombinant protein,the purified recombinant protein was used as antigen to immunize Japanese female big ear rabbits to prepare antibody,the specificity of the antibody was verified by mouse tissues.The changes of physiological indexes of mice were detected by models with different irradiation doses or irradiation methods;Western blot was used to detect the dynamic changes of BPIFA2 molecule relative expression in irradiated mice.Results 1.BPIFA2 in parotid was successfully detected by Western blot,but not detected in thymus,liver and kidney.2.Serum BPIFA2 levels in mice were increased at 1h after irradiation of 0Gy(control),0.5Gy,1Gy,2Gy,4Gy,6.5Gy(sublethal)and 10Gy(lethal),it decreased 12 h after exposure.3.BPIFA2 levels were increased again at 3d after lethal dose irradiation,which did not occur in sublethal dose and control group,it proved that the increase was related to the survival rate.4.After local irradiation with lethal dose,BPIFA2 increased rapidly,irradiation area is proportional to the increased.Conclusion 1.Polyclonal antibody against BPIFA2 was successfully prepared.2.Serum BPIFA2 levels in mice were increased at early-stage after irradiation,suggesting that it is a sensitive and reliable early indicator of radiation damage,which can be used to identify radiation injury personnel in the early stage of nuclear accident.3.Three days after lethal dose irradiation,serum BPIFA2 level increased significantly,suggesting that it can be used as a biomarker to accurately identify lethal radiation injury.4.The increase of BPIFA2 expression level after irradiation reflects the overall injury level,and has nothing to do with the irradiated part of the mice.Figure 16;Table 14;Reference 87... |
