Synthesis And Glucose Sensing Performance Of Hierarchical Micro Nanoarrays

With the development of human society and the improvement of living standards,the diabetes has become one of the four chronic noninfectious diseases.For diabetics,accurate monitoring of blood glucose levels and control of daily glucose intake are of great significance.At present,most glucose biosensors on the market are composed of immobilized enzymes,such as glucose oxidase,glucose dehydrogenase or other similar enzymes on different substrates.However,the enzyme sensor is easily inactivated,difficult to store,and the steps of fixing to the sensor surface are cumbersome and complex.Therefore,the enzyme free glucose sensor with low price and convenient operation has attracted people’s attention.With the vigorous development of new nanomaterials,many transition metals show excellent catalytic activity for glucose due to their nanosized characteristics and the synergy between transition metals,which has become a research hotspot in the field of enzyme-free glucose sensors.In addition,the performance of the sensor largely depends on the morphology of the nanomaterials used in the electrode structure.The preparation of nanomaterials with special morphology can effectively increase the surface area of the electrode and improve the performance of the sensor.Based on the above research,we use the gas-liquid interface self-assembly method to construct a single-layer colloidal crystal template,and combined with electrodeposition to obtain Ni bowl shaped micro nanoarray,and then take this as the substrate to regenerate new thin film materials to obtain the hierarchical micro nanoarray structure in this paper.The morphology,structure and composition were characterized by SEM,XRD,XPS,TEM and EDS,and the catalytic performance for glucose solution in alkaline environment was studied on CHI 660E electrochemical workstation.The specific research contents are as follows:（1）Synthesis of Co₉S₈/Ni hierarchical micro nanoarrays and their glucose sensing property:The two-dimensional polystyrene colloidal sphere template was prepared by gas-liquid interface self-assembly method,and the Ni bowl array was prepared by constant current deposition method of two electrode system.Finally,Co₉S₈nanosheets were deposited on the Ni bowl array by cyclic voltammetry of three electrode system to obtain Co₉S₈/Ni graded micro nanoarray.By adjusting the deposition temperature and time,the concentration of Ni Cl₂·6H₂O,Co（NO₃）₂·6H₂O and CS（NH₂）₂,a Co₉S₈/Ni hierarchical micro nanoarray with the best morphology was synthesized for the detection of glucose.It is found that the sensitivity of the synthesized Co₉S₈/Ni hierarchical micro nanoarray electrode to glucose biosensor is987.8μA?m M^-1?cm^-2,with good anti-interference performance.（2）Synthesis of Ni-Co-S/Ni graded micro nanoarrays and their glucose sensing performance:Ni-Co-S/Ni hierarchical micro nanoarrays were successfully prepared by gas-liquid interface self-assembly and two-step electrodeposition.Using the prepared colloidal sphere template as the substrate,Ni bowl array was synthesized by galvanostatic deposition of two electrode system.Finally,Ni-Co-S nanoflakes were prepared on Ni bowl substrate by cyclic voltammetry of three electrode system.The morphology of the Ni-Co-S/Ni hierarchical micro nanoarrays was observed by SEM,which showed an ordered hexagonal dense packing structure in a large area.Meanwhile,the sensing performance of CHI 600E electrochemical workstation for glucose was tested.The results indicate that the sensitivity of the electrode to glucose could reach 1210.1μA?m M^-1?cm^-2,also has good anti-interference and low detection limit.（3）Synthesis of Mn Co₂O₄/Ni hierarchical micro nanoarrays and their glucose sensing property:Ni bowl array was prepared by gas-liquid interface self-assembly method and constant current deposition method of two electrode system.Subsequently,Mn Co₂O₄nanoplates were deposited on Ni bowl substrate by cyclic voltammetry of three electrode system.By adjusting the deposition conditions,Mn Co₂O₄/Ni hierarchical micro nanoarrays with the best morphology and catalytic performance were obtained,and its glucose catalytic performance was studied.The results showed that the sensor based on Mn Co₂O₄/Ni hierarchical micro nanoarray had a sensitivity of 1438.3μA?m M^-1?cm^-2to glucose,with excellent anti-interference performance.