Preparation And Application Research Of Hydrogen Rich Water Based On Bio-surfactant

In recent years,hydrogen-rich water has been found to have significant antioxidant properties,especially in its application in agriculture,with a significant impact on plant growth.The application of hydrogen-rich water in the agricultural field is one of the current research focuses in modern agricultural development.However,the solubility of hydrogen gas in water is very limited under normal temperature and pressure conditions,making the preparation of hydrogen-rich water challenging.The preparation of high-concentration hydrogen-rich water at normal temperature and pressure has become a major bottleneck in the development of hydrogen water agriculture technology.The amphiphilic nature of biosurfactants allows them to have certain solubilizing effects.It has been proven that biosurfactants can increase the absorption rate of fertilizers in plants,enhance plant antibacterial abilities,and promote plant growth.They have the advantages of being renewable and naturally degradable,and are widely used in the field of agriculture.This study investigated the technology and equipment for the preparation of hydrogen-rich water based on biosurfactants at normal temperature and pressure,and conducted optimization research on the biosurfactants used in the preparation process of hydrogen-rich water for agricultural purposes.The research content and results are as follows:(1)The design,manufacturing,and optimization methods of the agricultural hydrogen-rich water preparation equipment were studied.The optimization methods included the adjustment of key parameters such as hydrogenation time and hydrogenation flow rate to ensure the accuracy and reliability of the experimental results.The results showed that in 200 m L of water,the best hydrogen solubility of 1517 ppb could be obtained by introducing pure hydrogen at a rate of 20 m L/s for 40 minutes,verifying the feasibility of the cyclic hydrogenation preparation device for hydrogen-rich water.Based on this,the concept of a continuous flow cyclic hydrogenation hydrogen-rich water preparation device was proposed.(2)The solubilization effects of different concentrations of biosurfactants on hydrogen-rich water were studied,and a single exponential decay model was used for fitting.The results showed that an appropriate amount of biosurfactants could increase the solubility of hydrogen gas without significantly affecting the total dissolution time,which remained around 10 hours.Trehalose lipids showed the optimal solubilization effect,with an increase of 17.51% in dissolved hydrogen compared to the control group at a concentration of 6 times the critical micelle concentration(CMC)of 1080 mg/L.Rhamnolipids ranked second with a 14.25%increase,but the optimal concentration for its addition was 2 times the CMC(240 mg/L),requiring a smaller amount compared to trehalose lipids.Sophorolipids had the weakest solubilization effect,with only a 6.42% increase in initial hydrogen solubility at a concentration of 1 times the CMC(180 mg/L).After reaching the CMC concentration,sugar lipid micelles would form different shapes,such as lamellar and vesicular structures,resulting in different hydrogen solubility capacities.When the composite liquid consisted of rhamnolipids at a concentration of 334.56 mg/L,sophorolipids at 237.4 mg/L,and trehalose lipids at 1197.24 mg/L,the highest hydrogen solubility increase of 21.14% was observed compared to the control group.This indicates that different types of biosurfactants,when mixed in certain proportions,exhibit a synergistic effect on increasing hydrogen solubility,which is also related to the different hydrogen solubility capacities of micelles in different shapes.(3)Hydrogen-rich water based on biosurfactants of different types was used for the cultivation of seedlings,revealing the impact on plant growth and antioxidant capacity.Seedlings cultivated with biosurfactant hydrogen-rich water showed varying degrees of improvement compared to pure hydrogen-rich water.Rhamnolipid hydrogen-rich water had a more pronounced promoting effect on soybean germination and growth compared to other biosurfactant groups.It increased the germination rate by 4%,the ratio of fresh to dry weight of seedlings by 5.4%,and the height of harvested plants by 12.8%.On the other hand,sophorolipid hydrogen-rich water had a more significant promoting effect on wheat,increasing the germination rate,seed water absorption rate,and the ratio of fresh to dry weight of seedlings by 3%,5.6%,and 16.1%,respectively.Although the overall growth of soybeans and wheat using composite solution-prepared hydrogen-rich water was relatively poor,their ascorbic acid and total phenol content were significantly higher compared to the hydrogenrich water prepared with a single biosurfactant.The composite solution hydrogen-rich water showed a 13.57% increase in DPPH(2,2-Diphenyl-1-picrylhydrazyl)radical scavenging capacity for soybeans,and a 12.16% increase for wheat compared to the pure hydrogen-rich water group.Hydrogen-rich water with an appropriate concentration and type of biosurfactant can promote the growth of seedlings and enhance their antioxidant capacity,thus improving their nutritional quality.In summary,this study investigated the technology and equipment for preparing hydrogenrich water using biosurfactants at normal temperature and pressure,as well as its application in the field of agriculture.It provides theoretical and technical support for further optimization of the preparation process of green agricultural hydrogen-rich water and the development of hydrogen-rich water production in industrial,medical,and other fields.