The Effects Of Single-walled Carbon Nanotubes Loaded Moroxydine Against Gcrv In Vitro

Grass carp(Ctenopharyngodon idella)is an important aquaculture specie of freshwater aquaculture,its production ranks the first in the "Four fish",but the development of the industry plagued by grass carp hemorrhage.Currently,the grass carp hemorrhage caused by grass carp reovirus(Grass carp reovirus,GCRV)has not yet found an effective prevention of drugs and methods,which became a bottleneck in the development of grass carp rearing.Currently,the production and practice are often used to prevent and treat immune pathways,in feed or antiviral herbs and animal extracts derived from an immune enhancer and to improve the breeding environment,taking comprehensive measures and so on.Prevention through immunization pathway which is considered to be the most effective way,however,in terms of cost control immune pathways and many other issues,such as the injection of inactivated vaccine technical complexity of the operation,heavy workload,the economic costs are high;topical or oral administration way due to the restrictions by the barrier membrane of fish its drug dissolution and absorption of a small amount of the drug into the cell concentration is often less than effective dose.Therefore,the development of an effective drug carrier to overcome the transport drugs across the membrane barrier problem in the treatment of viral diseases key grass.Carbon nanotubes(CNTs)as an vital one-dimensional nanomaterials,because of its unique tubular structure,special physical and chemical properties,large specific surface area,excellent transmembrane capabilities and more can be modified sites,targeted drug delivery system and in building long-circulating drugs biomedicine has good prospects and potential.However,carbon nanotubes are chemically inertness,almost insoluble in water and common organic solvents,which hinders researchers to explore their scientific research and applications,and numerous tests showed better solubility of CNTs,its toxicity is lower.In view of this,the research methods nanotubes by chemical modification and functionalization of the surface modification process to improve its biocompatibility,enhance its solubility,lower toxicity and expand the application range and a detailed study of renal cell CNTs on grass carp(Ctenopharyngodon idella kidney,CIK)toxicity.On this basis,further covalently linked to antiviral drugs-the virus spirit,to build a new type of carbon nanotube drug loading system and at the cellular level to study the drug loading capability,antiviral activity and intracellular distribution.In this study as a starting point to carry out different single-walled carbon nanotubes cytotoxicity,functional modification and its position as a drug carrier in vitro antiviral activity of research work,and obtained the following results:(1)In the cytotoxicity assay,we found that the original single-walled carbon nanotubes on the toxicity of CIK cells,and mixed acid oxidation treatment of SWCNTs,SWCNTs in increased water solubility and dispersion,while also reducing its Cytotoxicity to CIK cells.And these two kinds of SWCNTs showed a dose-time-dependent relationship to the toxicity of CIK cells.(2)SWCNTs cytotoxicity test results are consistent with other researchers’ findings in using mammalian cells as a model in the same conclusion,72 h LC50 of original and oxidized SWCNTs on CIK cells were 46.86 μg/mL and 203.21 μg/mL;SWCNTs as drug carriers can efficiently covalently linked moroxydine at its surface,its complexes content of up to 30.2%.(3)Set out in comparison with SWCNTs virus spirit and free virus spirit processing CIK cells,intracellular drug content was found,SWCNTs efficiently,a large amount of the drug molecule to the cell membrane.(4)The in vitro antiviral activity test showed that single-walled carbon nanotubes used as a carrier may be lower drug doses,increasing activity of infected cells and enhance the therapeutic index drugsThe results showed that single-walled carbon nanotube is of low toxicity on CIK cells,after modification by the functionlization it can be used as an ideal drug carrier for transport the drug molecules into the cells and for the treatment of viral diseases of fish.