| Green tea leafhopper(Empoasca flavescens)is one of the most destructive pests for tea plant,which causes significant economic loss in most tea grown countries in Asian.Currently,this pest is mainly controlled by harmful chemical insecticides.However,the over-use of chemical pesticides is environmental unfavorable.Meanwhile,pesticide residue is also an import limit for tea export.Thus,it has been an urgent issue to discover some effective bio-pesticides and lower their costs.In this study,the transcriptome of the intestinal tracts and carcass of the green tea leafhoppers were sequenced by Illumina sequencing technique.Investigation showed that over 6.309 Gb and 6.269 Gb Clean Data were obtained,respectively.De novo sequence assembly resulted in 52,182 Unigenes with the average size of 848 nt.BLAST result showed that at least 566 digestion-related genes,224 detoxification-related and 288 immune-related genes were identified,respectively.In addition,the relatively expression level from some of these genes were verified by quantitative real-time PCR.Result showed that these genes were expressed more in intestine than in carcass.Bacillus thuringiensis is one of the most popular bio-pesticide.In order to illustrate the physiological basis of Cry’s low toxicity against green tea leafhopper,Cry1Ac and Cry11Aa were hydrolyzed by E.flavescens’membrane fraction and lumen protease in vitro.Result shows that both Cry1Ac and Cry11Aa can be hydrolyzed into activated mature peptides by gut lumen and membrane fraction of E.flavescens,respectively.Results from this work may provide a reference for the genomic information of E.flavescens and contribute to the further biological study of E.flavescens,which is of great significance to the development of new strategies for the management of this pest. |
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