Bioinformatics Analysis Of TaHMGB11 Gene Family And Preliminary Functional Identification Of TaHMGB11

Label free quantification in the early stage of the laboratory found that the content of high migration group protein(TaHMGB11-7A)in wheat leaves was significantly up-regulated under water stress,suggesting that TaHMGB11-7A may play an important role in the response of wheat to abiotic stress.In this paper,the sequences,gene structures and conserved motifs of TaHMGB family members of wheat were analyzed by bioinformatics technique.TaHMGB11 gene was cloned from wheat cultivar Yunong 211.The BSMV:TaHMGB11 vector was constructed by the method of barley stripe Mosaic virus-virus induced gene-silencing(BSMV-VIGS),and the transcriptional level of TaHMGB11 in wheat seedlings was inhibited by the transient expression of leaf to study the function of TaHMGB11 in the response process of abiotic stress of wheat.On the other hand,TaHMGB11-7A overexpression vector was constructed and transformed into Arabidopsis thaliana,and positive transgenic offspring were obtained.The main research results are as follows:1.Through genome-wide identification,we found 30 TaHMGB genes in wheat,which could be divided into four gene subgroups based on phylogenetic analysis,including 19,6,2,3TaHMGBs,respectively.These 30 TaHMGB genes were distributed on 18 out of 21 chromosomes of wheat,and there was no TaHMGB gene on chromosome 4A,4B and 4D.A total of 37 pairs of TaHMGBs were fragment replicates,indicating that gene replication is important for the expansion of the HMGB gene family.The expression of TaHMGB genes in wheat is tissue-specific and stress-specific,and its predictive function is mainly related to the hormone response and abiotic stress response in wheat.TaHMGBs gene is an important multifunctional gene family.2.By VIGS technique,we constructed the vector BSMV: TaHMGB11 and inoculated it on the leaves of wheat seedlings to study the performance of gene silencing plants under abiotic stress.We found that under drought,high temperature and high salt stress,BSMV:TaHMGB11 silent plants had higher plant height,larger leaves and more robust plants than the control plants.Leaves water content and total root length were also significantly higher than the control.These results suggested that TaHMGB11 may be extensively involved in the response to abiotic stress in wheat with negative regulatory effects.3.We constructed the p CAMBIA1301-TaHMGB11-7A overexpression vector and transformed it into Arabidopsis thaliana to obtain T1 positive progenies.The subsequent screening of pure progenies is in progress.4.The coding region length of TaHMGB11-7A gene was 486 bp.We cloned the full-length TaHMGB11-7A gene from wheat and constructed the TaHMGB11-7A-GFP fusion expression vector.Transient expression of the vector in tobacco leaves indicated that TaHMGB11-7A was localized in the nucleus.