Bioinformatics Analysis Of Wheat G2-like Transcription Factor Family And Identification Of Resistance-related Genes

Wheat often suffers from various biotic and abiotic stress,which seriously affect wheat growth.Golden2-like(GLK,G2-like)plays a crucial role in chloroplast development,fruit quality,biotic and abiotic stress,and plant senescence.In this study,bioinformatics analysis was used to identify G2-like transcription factors on the whole genome of wheat,and then the physicochemical properties of proteins encoded by candidate wheat G2-like transcription factors were analyzed.A phylogenetic tree was constructed using 87 G2-like genes in wheat,and the gene structure and conserved domain of G2-like genes in wheat was analyzed.The phylogenetic tree between different species was constructed using 59 maize and 87 wheat G2-like genes.The expression of G2-like gene family in wheat seedling under drought and high temperature stress was investigated,the results showed that G2-like genes were highly expressed under salt,drought stress and also strongly responded to ABA,IAA and GA hormone stresses.Some genes that may be related to wheat stress resistance were obtained,and the role of Ta3AG2-like19 in wheat stress was verified.The main results as following:1.A total of 87 wheat G2-like transcription factors were identified in the whole wheat genome.By analyzing the physicochemical properties of proteins encoded by wheat G2-like gene,it was found that the acid and base distribution of G2-like protein was uniform,and the molecular weight and isoelectric point of G2-like protein varied greatly.The secondary structure of G2-like protein was mainly composed of α helices and random coils,and the subcellular localization prediction showed that it was mostly located in the nucleus.2.Using 87 wheat G2-like proteins to construct a phylogenetic tree,87 members were divided into 14 groups according to motif analysis and gene structure analysis.Wheat G2-like genes in the same group showed similar motif structure and gene structure.In addition,molecular evolution analysis found that wheat G2-like related genes were mainly purified and selected in evolution,that is,tended to be conserved during evolution.3.The analysis of cis-acting elements indicated that the wheat G2-like gene is regulated by multiple stress response elements,and it was found that its upstream 2-Kb region contained 7 species(P-box,Sp Ⅰ,LTR,ABRE,MBS,TGA-element and AE-box)cis-acting elements related to the induction of stress resistance.There are 62 G2-like genes containing at least 5 binding sites for stress-resistant cis-acting elements.Among them,Ta3AG2-like19 contains the most binding sites for cis-regulatory elements,with a total of 18 binding sites.4.The tissue expression analysis of G2-like genes found that all genes showed differences in expression levels in different tissues,and most G2-like genes were expressed in leaves.Under drought,high temperature,and the combined stress of drought and high temperature,some G2-like genes had higher expression levels,and their expression patterns are basically the same.q RT-PCR verification found that the expression levels of Ta3AG2-like19,Ta3AG2-like20,Ta4AG2-like29 and Ta6AG2-like52 were significantly up-regulated under the five stresses.5.For the Ta3AG2-like19 gene,which has a significant response pattern to stress,an over expression vector and a gene editing vector were successfully constructed.Exploring the important role of Ta3AG2-like19 gene in wheat adversity stress would provide useful information for wheat improvement.