Screening Of MRNA And LncRNA Related To High Fecundity And Low Fecundity Of Chuanzhong Black Goat Ovary During Estrus By Transcriptome Sequencing

Ovary is an important organ that determines the fertility of goats.The regulation of ovarian function is a complicated process,which is regulated by many hormones and genes.Therefore,it is speculated that there may be some factors related to the difference of expression amount in ovaries.Currently,improving goat fertility is one of the most important goals of goat breeders,but the molecular mechanism regulating goat fertility is unclear.In this study,transcriptome sequencing technology(RNA-Seq),combined with bioinformatics analysis and other methods,was used to find the high-fecundity goats(≥3 fetuses,≥2 kidlets)and low-fecundity goats(≥3 fetuses,per Fetal single kidlet)m RNA and lnc RNA expression differences,explore their functions and signal pathways involved,reveal the molecular mechanism of related genes and lnc RNA in regulating the ovarian function of different fecundity goats during estrus.It provides a theoretical basis for the molecular and genetic mechanisms related to goat fertility,and also lays a foundation for the scientific selection and breeding of high-fecundity and low-fecundity goats.The main findings are as follows:(1)In this study,1353 differentially expressed m RNAs(P <0.05)and 168 differentially expressed lnc RNAs(P <0.05)were screened between low and high fecundity goats;m RNA PVALB may be related to steroid-producing cells,and its significant up-regulation may affect the function of goat ovary(P <0.01).In addition,significantly up-regulated(P <0.05)m RNA RXRG plays an important role in the breeding process of females,and can be used as a candidate molecular marker for female reproduction.The real-time fluorescence quantitative PCR(q RT-PCR)method was used to verify the randomly selected differentially expressed m RNA and lnc RNA,and the quantitative results were basically consistent with the sequencing results.(2)The differentially expressed m RNA of GO Term is mainly related to the multicellular biological process,plasma membrane composition and plasma membrane activity.It is speculated that during oestrus,the material exchange of ovaries of high fecundity goat is frequent and the cell activity is relatively vigorous.IL1R2 can be used as a candidate gene for goat fertility,and it is enriched in multicellular biological processes.Enrichment analysis of m RNA in KEGG signaling pathway showed that differentially expressed genes such as SRD5A2,LOC102191297 and LOC102171967 participated in the metabolic pathway of steroid hormone biosynthesis,which was related to animal fertility.(3)Enrichment analysis of lnc RNA target gene KEGG signaling pathway shows that lnc RNA target genes MYC,SMAD6 and BMP2 are enriched in the TGF-β signaling pathway.lnc RNA may affect the normal regulation of TGF-β signaling pathway by mediating the expression of MYC,SMAD6 and BMP2,thereby regulating the function of ovary.(4)Joint analysis of m RNA-lnc RNA showed that two differentially expressed lnc RNAs ENSCHIT00000005909 and ENSCHIT00000005910 regulated the function of ovaries and affected the number of lambs of goats by regulating the differentially expressed IL1R2.In this study,ovarian tissue was used as the experimental material.We analyzed the m RNA and lnc RNA that may lead to differences in goat kidlet number from the transcriptome level.Some key genes that affected goat reproductive performance were initially screened,and the molecules that caused goat fertility differences were described.The mechanism and research results provide a theoretical basis for explaining the genetic mechanism of goat multi-lamb trait,and also provide scientific guidance for breeding of high-fecundity and low-fecundity goats.