Genetic Map Construction And QTL Mapping Of Phenotypic Traits In Hippophae Rhamnoides

Hippophae rhamnoides is not only edible and medicinal,but also has a variety of uses.It has a very broad development and utilization prospects.Due to the weak research on the molecular genetic basis of H.rhamnoides,the traditional breeding method is still the main breeding methods.In order to promote the genetic basis of H.rhamnoides and accelerate the molecular breeding process,this study took the H.rhamnoides.subsp.mongolica(‘Wulangemu’)as the female parent and H.rhamnoides.subsp.sinensis(‘Wucixiong’)as male parent,using F1 hybrids as mapping population,the first genetic map of H.rhamnoides was constructed by using three molecular markers of SCoT,SRAP and SSR.The QTLs of some phenotypic characters of H.rhamnoides were located.The main conclusions are as follows:1.The best reaction system of SCoT-PCR was established.24 SCoT,78 SRAP and 8 SSR pairs of primers were screened for the construction of H.rhamnoides genetic map.2.There were 3742 polymorphic sites obtained by three molecular markers analysis,among which 2152 sites were detected by chi square test,including 5 isolation types: 768 lm × ll(1:1);760 nn × np(1:1);45 hk × hk(1:2:1);578 hk × hk(3:1);1 ef × eg(1:1:1:1).3.The genetic map of H.rhamnoides consists of 853 markers,which are divided into 21 linkage groups.The total map distance is 1545.4cM,the coverage rate is 79.8%,and the average map distance between the two adjacent markers is 1.9cM.Besides 21 linkage groups,it also includes 5 triads and 47 diploids.4.17 QTLs were obtained,6 QTLs were associated with seedling height,accounting for 16.4%-21.8% of the phenotypic variation separately,distributed in LG1(2),LG2(2),LG3(1)and LG16(1);11 QTLs were associated with ground diameter,accounting for 15.8%-22.0% of the phenotypic variation separately,distributed in LG1(3),LG2(7)and LG16(1).