Effect Of Magnetic Field On Escherichia Coli And Bacillus Subtilis And Construction Of Game Model

The rapid propagation of bacteria is prone to occur in the breeding of domestic livestock and poultry houses,which seriously affects the health and growth of livestock and poultry.At present,chemical and biological methods are mainly used for sterilization.Although these methods have a certain inhibitory effect on bacteria such as E.coli,they also have disadvantages such as environmental pollution and high cost.Magnetic field is a new sterilization method with high efficiency,low energy consumption and no pollution to the environment.It can well solve the problems caused by bacteria in existing livestock and poultry houses,and has high development prospects and promotion value.This article takes Bacillus subtilis and Escherichia coli as the main research objects,uses the Gompertz model to construct a growth curve,and combines the plate counting method to analyze the influence of the magnetic field under different parameters on the growth of Bacillus subtilis and Escherichia coli,and explores the static magnetic field on the growth of Escherichia coli,The bactericidal effect of Bacillus subtilis.Using the Lotka-Volterra game model,construct the game model of the two kinds of bacteria in the forward and the adversity situation.The fractal simulation test of bacteria through the magnetic field provides a good experimental and theoretical basis for the influence of static magnetic field on the growth and development of bacteria and the prediction of bacterial growth.conclusion as below:1.The growth curves of Escherichia coli and Bacillus subtilis were determined by spectrophotometry,and the Gompertz model was used for fitting,and its prediction effect was better.2.Study the inactivation effects of magnetic fields under different parameters on Bacillus subtilis and Escherichia coli.The results show that the lethality trend of Escherichia coli and Bacillus subtilis after magnetic field treatment increases with the increase of field coil current,treatment time,and magnetic induction intensity.Larger increases,and decreases with the increase of the magnetic field spacing.Among them,the treatment time has the greatest correlation with the lethality of Escherichia coli.The lethality of Escherichia coli with the same magnetic induction intensity produced by different currents and different magnetic field spacing is basically the same.When the magnetic induction intensity is 70 m T,the average lethality rate of Escherichia coli increased from 9.03% to 48.69% when the treatment time is 1h to 6h.Under the same treatment,the lethality rate of Bacillus subtilis ranges from 7.30% to 43.55%;at 160 m T magnetic induction Under the strength,when the treatment time is 6h,the average lethality of Escherichia coli reaches the maximum,which is 66.45%,and the average lethality of Bacillus subtilis is 59.31%.3.Spread the E.coli and Bacillus subtilis liquid before and after the magnetic field treatment on the same petri dish,and count the plates to obtain the data,and use the Lotka-Volterra game model to obtain the interaction coefficient of the two bacteria.In the good luck game,a12=0.4880;a21=-0.3974,under the adversity game,a12=0.1555,a21=-0.012,so that Bacillus subtilis inhibits E.coli.4.Use the DLA model to simulate on MATLAB.Under normal growth conditions,the fractal simulation fitting accuracy of Escherichia coli and Bacillus subtilis are R2=0.98821 and R2=0.973,respectively;after magnetic field treatment,the fractal simulation fitting accuracy of Escherichia coli and Bacillus subtilis are R2=0.93156 and R2=0.97097,respectively.