Mapping And Candidate Of BnaC.GL Gene In A Dominant Glossy Mutant In Rapeseed(Brassica Napus L.)

Oilseed rapeseed is the largest oil crop in China.The surface of rapeseed（Brassica napus L.）is covered with a layer of wax,which is the first barrier to resist drought and low temperature and other external environment.It is the result of long-term selection and evolution of rapeseed to adapt to external environment.A waxless mutant gly was found in the breeding material.F₁was obtained by crossing the homozygous parent of waxless and the wax parent of Zhongshuang 11,and F₂population was obtained by self-crossing in F₁generation.Genetic analysis of waxles trait was conducted according to the separation performance.BSA-seq was used to map the waxless traits.RNA-seq was used for transcriptomic analysis of waxless and waxy plants of two parents,F₂and near-isogenic line（NIL）.Genetic markers and linkage analysis were combined to conduct gene mapping analysis,and the results were as follows:1.According to the separation of F₁,F₂and near-isogenic characters from the homozygous glossy mutant gly and the wax-powder-free mutant Shuang11,the glossy mutant gly was inherited by single dominant gene.2.According to the segregation of F₁and F₂between the homozygous waxless single plant isolated from the waxless plant and the waxy variety Zhongshuang 11,Chi square analysis showed that the waxy trait of the cabbage waxless powder plant gly was a single gene dominant inheritance,and there was no cytoplasmic effect.3.In the candidate region,113 pairs of SSR primers and 235 pairs of In Del primers were designed,and 7 pairs of closely linked markers were screened out.The single plant exchange analysis was carried out using a large F₂population of gly×Zhongshuang 11recessive single plant,and the glossy gene BnaC.GL was located between markers IN121and SSR-2-304,There were 10 candidate genes in this region.4.A common differential expression gene BnaC 08g43810D was obtained by transcriptome analysis of plants without wax powder and with wax powder in parents,F₂and near-isogenic lines.The gene is located within the interlocking interval.The candidate gene BnaC08g43810D was verified by q PCR using plants with and without wax powder in near-isogenic lines.The differential expression of BnaC08g43810D in leaves of near-isogenic lines was consistent with transcriptomic analysis,and BnaC08g43810D was confirmed as the candidate gene.