| This study takes the fattening of northern winter mutton sheep as the research theme,and compares the differences between the traditional northern winter mutton sheep breeding mode and the two feeding methods after supporting the heat storage sheep house and high-energy diet,so as to provide a theoretical basis for improving the number of northern winter mutton sheep breeding and promoting the intensive mutton sheep breeding in northern pastoral areas.The experiment lasted for 68 days.The pre feeding experiment lasted for 8 days and the formal experiment lasted for 60 days.Four experimental groups were designed,with10 fattening sheep in each group.The sheep in test group I were fed with 120% of the metabolic energy requirement of fattening sheep in the heat preservation enclosure,the sheep in test group II were fed with 100% of the metabolic energy requirement of fattening sheep in the heat preservation enclosure,the sheep in test group III were fed with 100% of the metabolic energy requirement of fattening sheep in the open enclosure(simulating the conventional enclosure in the pastoral area),and the sheep in test group IV were fed with 120% of the metabolic energy requirement of fattening sheep in the open enclosure.During the experimental period,each experimental group was fed in an unrestricted way,and ensured to provide sufficient and clean drinking water and keep the enclosure clean.The test results are:In terms of production performance,in the middle of the experiment,the weight of fattening sheep was not significantly affected by the enclosure environment and dietary energy level(P > 0.05).At the end of the experiment,affected by the enclosure environment,the weight difference of fattening sheep was very significant(P < 0.01)×Energy interaction had no significant effect on the body weight of finishing sheep(P >0.05).Similarly,affected by the enclosure environment,the average daily gain of fattening sheep was significantly different(P < 0.01)× The effect of energy interaction on the average daily gain of finishing sheep was not significant(P > 0.05).For the average feed intake,the difference was significant(P < 0.05)under the influence of enclosure environment,which was affected by energy and enclosure × The effect of energy interaction was not significant(P > 0.05).During the whole experimental period,the feed weight ratio was affected by the enclosure environment,dietary energy and enclosure × The effect of energy interaction was very significant(P < 0.01).In terms of rumen fermentation,the enclosure environment had a significant impact on rumen fluid p H,rumen fluid NH3-N and acetic acid / propionic acid of volatile fatty acids(P < 0.05).The change of dietary energy level had a significant difference on acetic acid and butyric acid in volatile fatty acids.The overall data analysis showed that in the heat storage sheep house,the fattening sheep fed with 120% metabolic energy diet were better than those fed in open enclosure.In terms of blood metabolome,416 differential metabolites were screened out,and the main metabolites were glycine and L-phenylalanine(s)-α-Amino group-β-Phenylpropionic acid,4-d-hydroxydihydrosphingosine,4A hydroxytetrahydrobiopterin,5,6-dihydrouracil,3-indoleacetonitrile,D-proline,etc.the metabolic pathways involved depend on amino acid degradation,glycine,serine and threonine metabolism,tryptophan metabolism,arginine and proline metabolism,sphingolipid metabolism,primary bile acid biosynthesis,aminoacyl t RNA biosynthesis and ABC transporter metabolism.Combined with the relative quantitative mean of metabolites in each group,it was found that the metabolism of test group III and test group IV was more vigorous.Conclusion: the effect of fattening mutton sheep fed in heat storage sheep house is better than that of cold season fattening sheep fed by traditional feeding method in North China. |
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