| Vigorously developing alfalfa artificial grassland is an important way to solve the contradiction between grass and livestock,and it is also an urgent need to maintain the safety and stability of grass and livestock products in China.However,Chinese alfalfa is mainly planted in poor soil and poor natural environment.How to improve the overwintering rate has become a hot issue concerned by growers and researchers.Based on this,this paper treated alfalfa artificial grassland in Horqin Sandy land with different phosphorus fertilizers,such as no phosphorus application,low phosphorus and constant phosphorus,combined with overwintering rate,number of overwintering buds,soluble sugar,starch,soluble protein and root C,N,P before and after overwintering in three parts of root neck,main root and lateral root.The effects of phosphorus fertilizer on the overwintering and root physiological characteristics of different varieties of alfalfa were studied to provide the basis for the management of alfalfa phosphorus fertilizer in Horqin sandy land.The results show that:1The overwintering rate of alfalfa showed different changes due to different varieties and phosphorus application rates.The wintering rates of Joneng 401,3010,4030,Joneng salt-tolerant,Joneng 995,Knight 2,Knight T,Adina,WL358 HQ,WL168 HQ,Zhao dong alfalfa,and Jia sheng under normal phosphorus conditions were significantly higher than those without phosphorus and low phosphorus(P<0.05).The wintering rates of Joneng 801,Catro,Challenger,Consay,WL440 HQ,WL535 HQ,Gong nong No.1,and Canon under low phosphorus conditions were significantly higher than those without phosphorus and normal phosphorus(P<0.05).There was no significant difference in the wintering rates of Sberde,Galaxy,and WL298 HQ under different phosphorus conditions.The overwintering rate of Li shi was not significantly different from that of low phosphorus and normal phosphorus(P>0.05).Zhao dong alfalfa had the highest overwintering rate under normal phosphorus,and 3010 had the lowest overwintering rate under no phosphorus application.2The number of overwintering buds of alfalfa showed different changes due to different varieties and phosphorus application rates.The number of overwintering buds of 3010,4030 and Jia sheng was significantly higher than that of low phosphorus and normal phosphorus(P<0.05).The number of overwintering buds of Spider and WL440 HQ was significantly higher than that of no phosphorus and normal phosphorus(P<0.05).The number of overwintering buds of Ju eng 801,Castro,Da yin he,Challenger,Kang sai,WL525 HQ and Li shi was significantly higher than that of no phosphorus and normal phosphorus(P<0.05).There was no significant difference in the number of overwintering buds under different phosphorus application conditions(P>0.05).There was no significant difference in the number of overwintering buds under different phosphorus application conditions(P>0.05).The number of overwintering buds of 3010 was the highest under no phosphorus application,and the number of overwintering buds of Ju neng 801 was the lowest under normal phosphorus.3Overall variation of soluble sugars in alfalfa root neck,taproot and lateral roots.Before wintering,the soluble sugar content of the root neck gradually increased with the increase of phosphorus application level,and the soluble sugar content of the primary root and lateral roots decreased first and then increased with the increase of phosphorus application level.After wintering,the soluble sugar content of root neck and taproot decreased first and then increased with the increase of phosphorus level,while the soluble sugar content of lateral roots gradually increased with the increase of phosphorus application.The largest decrease in soluble sugar content in root neck was Gong nong No.1 under Changphosphorus,which decreased by 90.02 mg/g.The content of soluble sugar in the taproot decreased by 126.50 mg/g in the largest variety of Zhao dong alfalfa.The largest reduction in soluble sugar content of lateral roots was Changphosphorus Xia Gong nong No.1,which decreased by 140.30 mg/g.Before wintering,the starch content of root neck first increased and then decreased with the increase of phosphorus level,and the starch content of primary root and lateral root decreased first and then increased with the increase of phosphorus level.After wintering,the starch content of root neck,main root and lateral root gradually decreased with the increase of phosphorus level.The largest variety with a decrease in root neck starch content was Giant Energy 801 under constant phosphorus,which decreased by 79.89mg/g.The largest decrease in the content of primary root starch was Adina under constant phosphorus,which decreased to 89.63 mg/g.The largest reduction in lateral root starch content was Knight T under constant phosphorus,which decreased by 83.26mg/g.Before wintering,the soluble protein content of root neck gradually increased with the increase of phosphorus level,the soluble protein content of taproot showed a gradual decrease with the increase of phosphorus level,the soluble protein content of lateral roots decreased first and then increased with the increase of phosphorus level,and the soluble protein content of root neck,taproot and lateral root after wintering showed a gradual decrease with the increase of phosphorus level.The largest decrease in the soluble protein content of root neck was Adina under phosphorus,which decreased by 21.21 mg/g.The largest consumption of taproot soluble protein content was the salt tolerance of giant energy under low phosphorus,which decreased by 22.84mg/g.The largest consumption of lateral root soluble protein content was Zhao dong alfalfa under low phosphorus,which decreased by 23.94 mg/g.4Before overwintering,the root C content was significantly lower than that of other treatments(P<0.05)without phosphorus,the N content of roots was significantly higher than that of other treatments(P<0.05),and the root P content was significantly higher than that of other treatments(P<0.05)under low phosphorus.Under the condition of constant phosphorus,the root C content of Zhao dong alfalfa was as high as 58.21%.Under low phosphorus conditions,the root N content of Knight 2 was up to8.79 %.Under the condition of no phosphorus application,the root P content of Zhao dong alfalfa was as high as 0.42%.After wintering,the root C content was significantly lower than that of other treatments(P<0.05),and the N and P contents of roots were significantly higher than that of other treatments(P<0.05).Under the condition of no phosphorus,the root C content of Giant Energy salt-tolerant was as high as 59.04%,and under the condition of constant phosphorus,the root N content of Great Galaxy was up to 7.85%,and the root P content of Challenger and Kang sai was as high as 0.38%.5The gray correlation method was used to comprehensively evaluate and analyze the wintering rate of alfalfa and soluble sugar,starch,soluble protein and other indicators in different parts of alfalfa root system,and it was concluded that the six indicators with the highest correlation with wintering rate were P content before wintering of root system,C content before root wintering,P content after root overwinter,soluble protein content before wintering of root neck,C content after root wintering,and soluble sugar content before wintering of root neck. |
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