Effect Of The Halotolerant Yeast Meyerozyma Guilliermondii Inoculation On Growth And Salt Tolerance Of Tomato Plants

Soil salinity has become one of the major threats to agroforestry production and ecosystems worldwide,and as soil salinity continues to increase,the potential for plant growth to be subjected to salt stress is gradually increasing,seriously affecting landscaping and crop yields.How to efficiently improve the tolerance of plants to salt stress and how to improve the survival rate of garden plants and crop yield under salt stress is crucial for ecological construction and maintaining adequate food supply today.Some beneficial microorganisms are highly capable of improving plant tolerance to salt stress and crop yield.To investigate the alleviating effect of the salt-tolerant yeast Candida quinquefolium（Meyerozyma guilliermondii）on the salt stress of cherry tomato seedlings,in this study,dwarf cherry tomatoes were used as experimental subjects in a soil environment containing four Na Cl concentrations（0,100,200,300 m M）,and M.guilliermondii was used in symbiosis with cherry tomato plants to study The effect of M.guilliermondii on the salt tolerance of dwarf cherry tomatoes was studied.The main results were as follows.In terms of growth and development,the salt-tolerant yeast Meyerozyma guilliermondii infected tomato roots under different Na Cl concentration stresses.Inoculation with M.guilliermondii increased chlorophyll content,maintained higher photosynthetic rates,produced more light and pigments thereby improving photosynthetic mechanisms under salt stress and was a decisive factor in increasing biomass accumulation,thus M.guilliermondii positively regulated aboveground fresh weight,belowground fresh weight and fruit fresh weight of cherry tomato.Regarding the antioxidant system,at higher salinity（300 m M Na Cl）,the concentration of ASA in the leaves of tomato plants inoculated with M.guilliermondii increased significantly,but there was no significant effect on the concentration of GSH and proline.Inoculation of M.guilliermondii showed different effects on antioxidant enzymes.Inoculation significantly increased the activities of SOD,CAT and POD under100,200 and 300 m M Na Cl conditions.Under 300 m M Na Cl conditions,H₂O₂and MDA contents were significantly decreased after inoculation,indicating that inoculation with M.guilliermondii was effective in reducing plant damage and protecting plant cell membrane systems.In terms of osmoregulatory system,the levels of total soluble sugars and soluble protein contents in the leaves of tomato plants inoculated with M.guilliermondii increased,but there was no significant difference between treatments.This indicates that inoculation with M.guilliermondii played a role in improving soluble sugar and soluble protein biosynthesis under salt stress.Our results suggest that inoculation with the salt-tolerant yeast M.guilliermondii improved the salt tolerance of tomato plants by scavenging ROS,maintained osmotic balance,and promoted the growth of tomato plants by increasing chlorophyll levels and enhancing photosynthetic mechanisms.