Study On The Effect Of Flumesafen Stress On The Quality Of Vigna Umbellata Based On Metabolomics Technology

Pesticides are widely used in agricultural production activities to prevent and control diseases,pests and weeds.They are indispensable means of production in agricultural production and management.They can improve crop production and ensure the quality of agricultural products.However,the use of pesticides inevitably causes pesticide residues in agricultural products,which will pose potential risks to human health.To investigate the effects of the commonly used herbicide fluconazole(FSA)in the cultivation process of Vigna umbellata on the metabolites and metabolic pathways in Vigna umbellata and its plants,as well as the effects of FSA on the amino acids and quality of Vigna umbellata.Using Vigna umbellatas and their plants sprayed with different doses and times of FSA as experimental materials,high performance liquid chromatography ultraviolet detection(HPLC-UVD)technology was used to detect FSA residues in Vigna umbellatas and their plants;Simultaneously measure the protein,fat,moisture,ash content,sand yield,and 100 grain weight of Vigna umbellata;Using liquid chromatography-mass spectrometry(LC-MS)metabonomics technology,through principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),hierarchical clustering thermogram analysis and other multivariate statistical analysis methods,this paper discussed the growth of Vigna umbellata plants(Z-2-ZZ)without spraying pesticide FSA Compare the dynamic changes of small molecule metabolites and key metabolic pathways of FSA sprayed red bean plants(Z-2-ZZ-2)and sprayed Vigna umbellata plants(Z-2-GS-2)without spraying pesticide FSA,analyze the metabolic pathways and internal metabolic mechanisms of FSA in Vigna umbellata and its plants,accurately qualitatively and quantitatively analyze the 21 differential amino acids in Vigna umbellata(Z-2-GS-2),and analyze the impact of FSA stress on amino acids in Vigna umbellata,Provide theoretical basis for the rational use of FSA in the field of Vigna umbellata and the prevention and control of its harm.The results indicate that:(1)Residual analysis of FSA sprayed at different doses and times in Vigna umbellata and its plants.The residual range of FSA application in Vigna umbellata plants was 0.280～0.079mg/kg and 0.303～0.110 mg/kg,respectively,with a spraying rate of 0.15 m L/m~2once and twice;The residual range of 0.30 m L/m~2spraying once and twice in Vigna umbellata plants is0.300～0.120 mg/kg and 0.340～0.180 mg/kg,respectively;The residual range of FSA application in Vigna umbellata was 0.042～0.019 mg/kg and 0.045～0.029 mg/kg,respectively,with a spraying rate of 0.15 m L/m~2once and twice;The residual range of 0.30 m L/m~2applied once and twice in Vigna umbellata is 0.048～0.028 mg/kg and 0.089～0.053 mg/kg,respectively.(2)Quality analysis of Vigna umbellata under FSA stress at different doses and times.Spraying 0.15 m L/m~2FSA once and twice,as well as 0.30 m L/m~2FSA once,can lead to an increase in protein and ash content and a decrease in fat content in Vigna umbellata;Spraying0.30 m L/m~2FSA twice had a significant impact on the protein content,fat content,water content,100-grain weight,ash content,and sand yield of Vigna umbellata.The protein content,ash content,and sand yield increased,while the fat content,water content,and 100-grain weight decreased.Therefore,subsequent studies conducted metabolomics analysis on Vigna umbellata samples sprayed with 0.30 m L/m~2FSA twice.(3)Non-targeted metabolomics analysis of FSA stress on Vigna umbellata and its plants.By analyzing three sets of samples from Vigna umbellatas and their plants sprayed with 0.30m L/m~2FSA twice,106,37,and 63 differential metabolites were identified,and 5,6,and 5significantly different metabolic pathways were screened.The analyzed differential metabolites and metabolic pathways were mostly related to amino acids,indicating that FSA stress had a significant impact on amino acids in Vigna umbellatas.(4)Perform high-throughput targeted amino acid metabolomics analysis on Z-2-GS-2-vs-GS group Vigna umbellata samples.Accurately qualitatively and quantitatively identified 21 amino acids,including 7 sweet amino acids(Ala,L-Hyd,Lys,Met,Pro,Ser,Thr),4bitter amino acids(His,IIe,Leu,Tyr),and 8 medicinal amino acids(Arg,Asn,GIn,GIy,Leu,Lys,Met,Tyr).Under FSA stress,the content of sweet amino acids Asp and Glu increased,while the content of bitter amino acids Phe,His,and Ile decreased,indicating that FSA can lead to an increase in the sweet taste and a decrease in the bitter taste of Vigna umbellata.