Study On The Isolation And Extraction Of Metabolites From Clonostachys Rosea And Its Broad-spectrum Resistance And Growth Promoting Mechanism

Clonostachys rosea is a filamentous fungus that has been widely used in biological control,biological fermentation,biodegradation and bioenergy.As a biocontrol fungus,the role of its metabolites in the prevention and treatment is worthy of further exploration.In this study,we analyzed and identified the metabolites of Clonostachys rosea.Firstly,it was determined that the fermentation broth of Clonostachys rosea had an antibacterial effect,and then the best detection period of Clonostachys rosea was determined to be cultured to the fourth day.Finally,it was determined that Voacamine,cucurbitacin E and fucoxanthin were specific compounds that may have antibacterial effects.In the second part of this study,we studied the effects of Clonostachys rosea on tomato root growth and its mechanism.The results showed that Clonostachys rosea could promote the growth of tomato roots.It is worth noting that the addition of tryptophan enhanced its growth-promoting effect.The results also showed that tryptophan increased the abundance of metabolites in Clonostachys rosea spiralis,and auxin(IAA)and auxin-related metabolites accounted for the majority of differential metabolites in the presence of tryptophan.Studies have found that Clonostachys rosea can metabolize tryptophan to tryptamine(TRA)and indole-3-acetaldehyde(IAAId).These two compounds are used by Clonostachys rosea to produce IAA through the tryptamine(TAM)pathway,which is one of the main pathways for tryptophan-dependent IAA biosynthesis.IAA produced by Clonostachys rosea spiralis promoted tomato root growth.As far as we know,this is the first report that Clonostachys rosea synthesizes IAA through the TAM pathway.