Genotype Identification And Analysis Of Rotavirus In Calves In Some Areas Of Southern Xinjiang

Rotavirus is one of the important pathogens that cause digestive tract diseases in cattle.Sick cattle mostly show mental stagnation,dehydrated diarrhea and yellowish or grayish feces,which seriously affect the growth and development of calves and cause some economic losses to the cattle industry.Therefore,mastering the infection of BRV has certain guiding significance for its prevention and control.In order to identify the genotype of rotavirus infection in calves of large-scale beef cattle farms in some areas of southern Xinjiang and to grasp the molecular epidemiological characteristics of rotavirus in calves in southern Xinjiang,ELISA antigen detection and molecular epidemiological investigation of BRV in large-scale beef cattle farms in some areas of southern Xinjiang were carried out in this experiment:1.118 fecal samples of four large-scale beef cattle farms in some areas of southern Xinjiang were tested for Rota-Corona antigen ELISA in 2022,and 18 positive samples of bovine rotavirus were detected,and the antigen-positive rate of BRV was 15.25%,and the positive rates of bovine rotavirus in the four farms were 10.34%,18.52%,14.29% and 17.65%,respectively;2.Samples tested positive by ELISA for Rota-Corona antigen,their nucleic acids were extracted and amplified by RT-PCR with specific primers,nucleotide homology matching and construction of phylogenetic tree,and the results showed that the target bands of 1062 bp in size were obtained from 18 samples by RT-PCR amplification of VP7 specific primers(VP7-1/VP7/2)for RT-PCR with genotyping primers(VP7-1/G6-2)amplified 6 target bands of 500 bp,which were identified as rotavirus group A type G6 by sequencing and nucleotide homology analysis;RT-PCR with genotyping primers(VP7-1/G10-2)amplified 10 target bands of 715 bp,which were identified as rotavirus group A type G6 by sequencing and nucleotide homology analysis.Two target bands of 715 bp were obtained by RT-PCR with VP4-specific primers(VP4-1/VP4-2),which were identified as rotavirus group A,type G10,by sequencing and nucleotide homology matching analysis;Through the ELISA antigen detection and molecular epidemiological investigation of rotavirus in calves from large scale beef cattle farms in some areas of southern Xinjiang,the positive rotavirus infection rate,rotavirus genotypes,and molecular epidemiological characteristics of rotavirus in the region were grasped.The analysis revealed the presence of P[5] rotavirus in addition to two different genotypes,G10 and G6,in large-scale cattle farms in South Xinjiang.This study provides a reference for the prevention and control of diarrhea caused by rotavirus in calves in this region.