Drought Resistance Function Of Maize Glutathione S-transferase Gene ZmGST26

Maize is one of the crops with high planting and demand in our country.It is of great significance for safeguarding food,economic and ecological stability of our country.Drought severely restricts crop growth and development and more seriously threatens agricultural production.Glutathione S-transferase is an important multifunctional supergene family enzyme in the plant body and plays an important role in responding to abiotic stresses in plants.In this study,The down regulated gene ZmGST26 differentially expressed under drought stress was screened by the transcriptome sequencing data of maize inbred line“H8186”under drought stress.Clarifying the physiological and molecular mechanisms of its response to drought stress through a combination of plant genetic engineering and genetics.The main findings are as follows:1.Bioinformatic analysis of the ZmGST26 gene revealed that it encodes a protein of approximately 24.8 k Da,which is spatially moreαHelix predominates,does not have a transmembrane domain,has no signal peptide structure,belongs to hydrophilic proteins,subcellular localization prediction results localize to the nucleus,and interactors predicted to have the highest probability of interacting with the gst11protein.The promoter element analysis of the 2000bp upstream sequence of ZmGST26gene showed that there were a large number of action elements involved in the regulation of non Biotic stress.Through the analysis of gene expression levels in maize roots,stems,and leaves under different time periods of drought stress,the ZmGST26 gene can be expressed in roots,stems,and leaves,and shows a downward trend under drought stress.2.Constructed the prokaryotic expression vector pET-22b-ZmGST26 of the ZmGST26 gene,and expressed a protein of 24.8 k Da under IPTG induction;Successfully constructed a subcellular localization vector for this gene,and transiently transformed it into tobacco leaves through Agrobacterium mediated transformation.The results showed that the ZmGST26 protein mainly functions in the nucleus.It has been proven through yeast two hybrid experiments that there is an interaction relationship between ZmGST26 and ZmGST11 proteins3.Through genetic transformation 15 Arabidopsis thaliana lines with T₃generation ZmGST26 gene were obtained,and 22 maize plants with T₂generation ZmGST26 gene overexpression were obtained.The phenotypic,physiological and biochemical indicators and gene expression analysis of drought stress-related positive offspring plants under drought stress consistently showed that ZmGST26 genes reduced plant tolerance to drought stress.