Development And Experimental Study Of Field Mobile Phenotype Chamber

Plant phenotypic omics combination of functional genomics in the field of plant breeding and genetic technology,as a rapid and accurate method to achieve crop morphology and physiology parameters measurement,parsing genotype,environment,phenotypic relations,help the breeding attention of the whole crop growth cycle,then analysis and filter with high yield,resistance to salt,drought resistance and disease resistance of crop varieties.It is found that there are disadvantages of low efficiency,large error and high cost in traditional manual collection and analysis of phenotypic data.Therefore,it is very important to explore crop phenotypic characteristics and traits high throughput measurement technology.In view of the above problems,this paper develops a field mobile phenotype chamber,which is composed of Field movement of phenotype pods,Multi-sensor system and Phenotype analysis system to realize the key technology of integrated collection and analysis of field crop phenotype data.To achieve high-throughput continuous collection and analysis of crop phenotypic characteristics and physiological parameters.The specific work content is as follows:(1)Design and development of mobile phenotype cabin in fieldField mobile phenotypic cabin was developed,and a systematic design of phenotypic cabin frame system,five-dimensional data acquisition system,root system monitoring system and environmental control system was completed.The development and design of phenotypic cabin frame system and five-dimensional data acquisition system include hardware structure,motion control,positioning and motion path error,deviation correction and compensation.It has the functions of precise positioning,path planning,speed control and position feedback,etc.,with a high degree of automation.The development and design of root monitoring system include underground observation system,root window monitoring system,endoscopic image acquisition system and image acquisition and control,so as to realize non-damage,sustainable,high-frequency tracking observation and in-situ acquisition of soil root information.The development and design of the environmental monitoring system include the environmental monitoring device and the hardware control system to realize the real-time perception and control of the cabin environment;(2)Design of Multi-sensor systemMultiple sensor system was developed,complete hyperspectral,thermal infrared,RGB,multispectral and depth integration of multiple sensors,sensor control modular and information standardization study,multithreading,multi-channel phenotype image automatic acquisition and generate technical problems,in the "terminal" to realize the image acquisition of multiple sensors;(3)Design of Phenotypic analysis systemDeveloped the phenotypic analysis system,and completed the systematic design of client-side operating software system,server-side scheduling system and data management and analysis module.The design of the client operation software system includes the interface design of the client registration and login module and the multi-sensor acquisition and control module.The design of the server dispatching system includes the design of five-axis acquisition system and phenotypic cabin movement control,the design of multi-sensor acquisition control and the design of field environment and cabin environment control.Data management and analysis design include data loading and storage,data analysis and processing,and the processing of different data sources,which can realize the analysis of different phenotypic data;(4)Canopy phenotype acquisition test of wheatThe experimental study of wheat growth information acquisition was completed,and wheat experiment design,data acquisition,testing and analysis were carried out.The leaf area data,leaf area index(LAI)data,coverage data,seedling loss rate data,freezing injury data and canopy temperature data of wheat were extracted and analyzed.The analysis methods included correlation analysis and regression analysis to realize the interpretation of leaf area,leaf area index(LAI),coverage data,seedling loss rate,freezing injury situation and canopy temperature.The accuracy,rapidity,high efficiency and functionality of the mobile phenotypic chamber in the field were reflected and verified through the wheat growth information acquisition experiment.