Interaction Of Mammalian Early Innate Immune Development Genes With Microbiota

Innate immunity relies on the surface barrier and the cells and proteins inside the organism to achieve a wide range of adaptive and defensive effects,and its related genes are essential in embryonic development and mutual adaptation of the mother and fetus,and the colonization of intestinal microorganisms and their interaction with living organisms cannot be separated from the regulation of innate immunity genes.In order to investigate the regulatory mechanisms of innate immunity genes in mammals at the genome-wide level,six important mammalian species were selected for this study: mous,human,horse,pig,sheep,and cow.Based on comparative genomics,the main findings were obtained as follows:1.Based on the collection of the latest reported human innate immunity genes and the updated genomic data of six mammalian species: mouse,horse,pig,sheep and cattle for comparison,1674 innate immunity genes of mouse,1569 innate immunity genes of horse,1865 innate immunity genes of pig,2193 innate immunity genes of sheep and1473 innate immunity genes of cattle were screened,and a database of mammalian innate immunity genes was initially constructed.2,Comparative analysis of pre-embryonic transcriptome numbers of different developmental periods in cow,mouse,pig,and differentially expressed innate immunity genes were obtained after matching.The significant modules of milk,mouse and pig were obtained by WGCNA analysis,gene co-expression network construction and hub gene identification,and candidate central genes for early embryonic development in milk,mouse and pig were obtained.3.Microbial analysis of calf host liver transcriptomics revealed the regulatory mechanism of the liver under the influence of intestinal microorganisms,and identified IL-2 as an upstream regulator,which establishes a link between the intestine and the liver in the partnership of innate immune function.In summary,this study depicts a panoramic view of the network of mammalian innate immune genes and the early embryonic period,and provides a deeper discussion of the innate immune genes associated before and after the syncytial stage,providing important insights into the network regulation of innate immune genes in the early embryonic period,as well as demonstrating the major innate immune genes and associated biological processes under the influence of microorganisms,providing a basis for the cross-organ regulation of innate immune genes in response to microbial stimulation of the intestine.