| HSF transcription factor plays an important role in plant growth and development and response to stress.In order to reveal the role of HSF transcription factor in poplar growth and response to salt stress,this study used bioinformatics to analyze the characteristics of HSF family and detected their expression patterns.The function of salt stress related gene HSF4 was preliminarily explored using the genetic engineering.1.Thirty members of poplar HSF transcription factors were identified.Through phylogenetic analysis,these proteins were divided into three categories: A,B,C,and15 subcategories.The results of sequence characteristic analysis showed that the HSF members of poplar also have conserved DNA binding domain composed of 3 α-helices and 4 antiparallel β-folds.HSF were mainly localized in the nucleus.Gene structure analysis showed that 29 of the 30 HSF genes had only two exons and one intron.2.Gene co-expression networks centered on thirty poplar HSF genes were constructed and GO enrichment analysis was performed.Combined with transcriptome sequencing data,the response salt stress transcription factor HSF4 was screened,which was localized in the nucleus and had no self-activation activity.3.The overexpression and inhibitory expression vectors of HSF4 were constructed,and 4 overexpression and 6 inhibitory transgenic lines of HSF4 were cultivated.It was found that HSF4 significantly inhibited plant growth,which was manifested in the significant reduction of plant height,fresh weight,stem node number and leaf number,but significantly promoted the development of mature leaves,which was manifested in the significant increase of leaf area and petiole length.After salt stress treatment,the plant height,fresh weight,stem node number of HSF4 overexpression lines and the stem node length and fresh weight of mature leaves were greatly inhibited compared to WT and RNAi lines,indicating that HSF4 reduced the tolerance of poplar to salt stress. |
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