| Porcine Reproductive and Respiratory Syndrome(PRRS)is a highly contagious infectious disease,Caused by Porcine Reproductive and Respiratory Syndrome Virus(PRRSV),this disease can cause miscarriage in sows,stillbirth,mummified foetuses,and dyspnea and hyperthermia in pigs at various stages of development.The disease spreads rapidly and has a high incidence,with the most serious clinical symptoms after infection of pregnant sows and piglets within 1 month of age.The disease was first discovered in North America in 1987,and then gradually spread around the world,bringing huge economic losses to the pig industry and becoming one of the most important pathogens affecting the world’s pig industry.If the disease is diagnosed at the early stage of the disease,corresponding preventive and treatment measures can be taken in advance,which is of great significance for the removal of PRRSV in pig farms.The conventional PCR technique needs to determine whether the disease is infected according to the fragment size through electrophoresis and irradiation after amplification,which is costly and cumbersome.This project mainly combines PCR technology and test strip technology to amplify the target gene of PRRSV through PCR,and then uses the test strip to detect the disease.This method shorens the detection time,and can directly obtain the results through observation,without the need for photostat and electrophoresis apparatus,and reduces the detection cost.In this experiment,according to the PCR primers for PRRSV published by the World Organization for Animal Health(WOAH),two amplification primers were synthesized,a PCR detection method for PRRSV was established,and the annealing temperature and primer concentration during the PCR process were optimized.Based on the PCR products,the immunochromatographic test strip detection technique was established.fluorescent microspheres(FMs)was used to establish fluorescent microspheres immunochromatographic strip detection technology.The sensitivity of fluorescent microspheres as tracer is 100 times higher than that of ordinary immunochromatography. |
