Study On The Effects Of Nesfatin-1 On Oxidative Stress And Autophagy In Starved Mice

Bactrian camel has strong cold resistance,hunger resistance and other biological characteristics,can survive in harsh environment for a long time.In previous studies,Nesfatin-1 was found to regulate the efficiency of energy metabolism in the body and was expressed in numerous tissues of Bactrian camels.However,it is inconclusive whether Nesfatin-1 is associated with the unique energy metabolic properties of bactrian camels.In order to explore the regulatory mechanism of Nesfatin-1 on energy metabolism in the body,this experiment will study the effects of Nesfatin-1 on oxidative stress and autophagy in the body under the state of starvation.Methods: In this experiment,mice were used as research objects to establish a starvation model,which was divided into negative control group,24 h fasting group,48 h fasting group,Nesfatin-1+24 h fasting group and Nesfatin-1+48 h fasting group.Nesfatin-1 group was intraperitoneally injected 1.25 nmol/g Nesfatin-1 after 24 h and 48 h of fasting,respectively.The control group was intraperitoneally injected with the same dose of PBS buffer.The levels of reactive oxygen species(ROS)in the liver,Malonaldehyde(MDA)and Nesfinin-1 in serum of mice were measured by chemical fluorescence and ELISA methods,respectively.Then quantitative real-time PCR and Western Blot were used to detect Beclin-1,LC3,P62 m RNA and protein expression levels in the brain tissue of each group of mice,respectively.Results:(1)After 24 h and 48 h of fasting,serum Nesfatin-1 levels in mice were significantly lower than those in the control group(P<0.05);(2)Compared with the negative control group,the levels of ROS and MDA were highly significantly increased(P<0.01),the expression levels of Beclin-1 m RNA and protein were highly significantly increased(P<0.01),the expression levels of LC3 m RNA were increased(P<0.05),and the expression levels of P62 m RNA and protein were highly significantly decreased in the 24 h fasting group and the 48 h fasting group(P<0.01);(3)After intervention with Nesfatin-1,compared with the 24 h fasting group,ROS,MDA levels and Beclin-1m RNA and protein levels in the Nesfatin-1+24 h fasting group mice were highly significantly reduced(P<0.01),LC3 m RNA levels were highly significantly reduced(P<0.01),and P62 m RNA and protein expression levels were significantly elevated(P<0.05);compared with the 48 h fasting group,ROS,MDA levels and Beclin-1 m RNA and protein levels were highly significantly reduced(P<0.01),LC3 m RNA levels were highly significantly reduced(P<0.01),and P62 m RNA and protein expression levels were upregulated in the Nesfatin-1+ fasting 48 h group mice.Conclusion: Starvation can increase the levels of oxidative stress and autophagy in mice,and after Nesfatin-1 intervention,the levels of oxidative stress and autophagy in mice were significantly decreased,suggesting that Nesfatin-1 may reduce starvation-induced damage by inhibiting oxidative stress and autophagy in the organism.This study provides an important experimental basis for further research on the mechanism of Nesfatin-1 regulation of energy metabolism in the body,in order to finally provide a new idea for the research of metabolic diseases prevention and treatment.