Excavation Of Genes Related To Organ Development Of Prunus Sibirica Flower

The Prunus sibirica It is an important economic tree species in the northern region of our country.The application prospects are wide and the development potential is very great.At the same time,the Prunus sibirica is the pioneer tree species for soil and water conservation and windbreak and sand fixation in arid and semi-arid areas,which has high ecological value.However,the yield of Prunus sibirica was low and unstable due to pistil abortion and freezing damage in flowering period,etc.Further exploration of the genes related to flower organ development could provide an important theoretical basis for the yield increase and quality improvement of Prunus sibirica.In this study,the cell anatomical characteristics of flower bud differentiation of Prunus sibirica were observed by paraffin section technique.Through transcriptomics,we screened the genes involved in the regulation of organ development in Prunus sibirica blossom,which provided an important theoretical basis for the analysis of the molecular regulation mechanism of organ development in Prunus sibirica blossom.The main results are as follows:1.The results of paraffin section showed that the flower bud development process of Prunus sibirica CT was divided into 6 stages,namely,undifferentiated stage,early differentiation stage,calyx differentiation stage,petal differentiation stage,stamen differentiation stage and pistil differentiation stage.Compared with the flower bud differentiation process of Prunus sibirica CT and BT,it was found that the main difference was the differentiation of female and stamen.BT has no stamen but has multiple pistills.The formation stage of the female and stamen primordia of Prunus sibirica CT on August 25 and Prunus sibirica BT on September 5 is the key stage of morphological difference between CT and BT.2.Transcriptome sequencing showed that 2459 differentially expressed genes were detected,of which 1367 were up-regulated and 1092 down-regulated.GO enrichment analysis of differentially expressed genes showed that they were mainly concentrated in biological processes such as cellular process,metabolic process,cell anatomical entity and binding,and catalytic activity.The results of KEGG enrichment analysis of differentially expressed genes showed that the enrichment pathways included flavonoid biosynthesis,biosynthesis of stilbenes,heptane diphosphate and gingerol,plant and pathogen interaction,secondary metabolite biosynthesis and hormone signal transduction,which indicated that the metabolic process and secondary metabolites were closely related to the development of Prunus sibirica flower organs.3.According to the functional annotation of differentially expressed genes in the flower buds of Prunus sibirica,4 MADS-box transcription factors and 43 differentially expressed genes related to plant hormones were found to be related to flower organ development.Relevant studies have found that ABCDE model B gene,IAA signal transduction Aux/IAA family gene,ABA signal transduction PP2 C gene,SA signal transduction TGA gene are related to flower organ development.Therefore,it is speculated that the MADS-box gene family of flower development model B gene Ps MADS9,The IAA signal transduction pathway IAA10,IAA4/5 and Pa18184 genes,ABA signal transduction pathway HAB1 gene and SA signal transduction pathway TGA9 and PRB1 genes in plant hormone-related genes are related to organ development of Prunus sibirica flower.Transcriptome sequencing results were verified by q RT-PCR.The results are basically consistent with the transcriptome results.Based on the analysis of paraffin sections and transcriptome sequencing,this study initially screened out genes related to the organ development of Prunus sibirica flower,providing a theoretical basis for the analysis mechanism of the organ development of Prunus sibirica.