Transcriptome Study Of Rice Response To Rice Blast Infection And Mining Of Disease Resistance-related Genes

Rice is one of the world’s most significant food crops.Rice production has been significantly hampered by the emergence of different biotic and abiotic stressors.Rice blast,caused by the rice fungus Magnaporthe oryzae,is one of the most damaging diseases in rice production,and it can affect the plant at any point of its reproductive cycle and in any section of the plant.Rice blast infection is most vulnerable at the seedling stage,and severe damage might result in seedling death.Studying the mechanism of disease resistance and genetic changes in linked metabolic pathways in rice under R.paddy infestation is thus of considerable scientific value.Using TY,a broad-spectrum durable resistant material,and CO39,a highly susceptible variety of rice plague,as experimental materials,the physiological level,transcriptome level,and QTL localization of two different resistant rice recombinant self-incompatible lines were investigated.The goal of the study was to look into the molecular mechanism of rice resistance to rice blast by looking at physiological traits of rice materials in response to blast fungus,the transcriptome combined with QTL localization analysis,to further explore resistance genes that can be used for genetic improvement,to provide a reference for screening and breeding broad-spectrum durable disease resistant varieties.The main findings are as follows.(1)The physiological response mechanisms of TY and CO39 to blast fungus were discovered to be distinct,with TY being able to maintain the stability of important functions in the body and CO39 being more capable of scavenging hydroxyl radicals.CO39 leaves,on the other hand,showed larger levels of malondialdehyde and hydrogen peroxide,as well as higher levels of soluble sugar,proline,reduced glutathione,and antioxidant enzyme activity for scavenging excess reactive oxygen species and protecting cell membranes from oxidative damage.CO39 was able to amass more abscisic acid,Cytokinin,Jasmonic acid,and Salicylic acid to strengthen its antioxidant capacity,as well as a considerable increase in the stimulating hormone Auxein,as a result of the increase in rice endogenous hormone levels.(2)Rice leaves infested with RNA-Seq technology had their gene expression levels examined,and differentially expressed genes were identified and studied using the GO and KEGG databases.The researchers discovered a total of 10,542 differently expressed genes between TY and CO39,with metabolic pathways such as phytohormone signaling,amino acid synthesis,mitogen-activated protein kinase signaling pathway,and carbon metabolism being considerably enriched.Further investigation revealed that the lower Auxein content in TY leaves compared to CO39 was due to significant down-regulation of genes encoding AUX protein and receptor protein TIR1 in the plant hormone signal transduction pathway;the lower proline content in TY was due to significant down-regulation of genes encoding pyrroline-5-carboxylic acid reductase in the biosynthesis of amino acids pathway;and the up-regulation of genes encoding acetyl coenzyme A The ability of TY to sustain normal growth and development is due to upregulation of genes encoding acetyl coenzyme A and succinyl coenzyme A in the carbon metabolism pathway.(3)Rice blast inoculation identification of the TY/CO39 F7 RILs population revealed five resistance genes(QTLs)on chromosomes 2,6,7,8,and 12.q DS_F12 was discovered on chromosome 12 between WY1235 and WY1217,near the mitosis of chromosome 12,and is very likely to be a new rice blast resistance gene,and it’s gene function needs to be further verified.