Study On The Effect Of Nevadensin To Alleviate Fatty Liver In Chickens

In recent years,the scale and intensification of poultry farming has gradually expanded,resulting in the increasing incidence of fatty liver syndrome(FLS)in chickens.Fatty liver causes a decrease in feed conversion,production and reproductive performance,and an increase in mortality,which not only endangers the health of birds,but also causes serious economic losses to the poultry industry.The liver plays a key role in the lipid metabolism of birds.When poultry liver lipid metabolism is disturbed,fatty liver is often induced.There is no effective drug for the prevention or treatment of fatty liver syndrome in chickens.In recent years,plant extracts have shown positive effects as feed additives in the prevention and treatment of fatty liver syndrome in chickens.Therefore,it is of great practical importance to find and screen new plant extracts that are effective in preventing and treating fatty liver syndrome in chickens.Nevadensin is a natural flavonoid extracted from the traditional Chinese herb Lithophanum,which is widely available and has remarkable efficacy in hypotension,anti-inflammation and antibacterial.Currently,the regulatory effects of nevadensin on hepatic lipid metabolism have not been reported at home or abroad.To investigate its role in hepatic lipid metabolism,the present study firstly investigated the regulatory effects of nevadensin on high-fatinduced hepatocyte lipid metabolism in Hep G2 hepatoma cells treated with oleic acid and palmitic acid(OA/PA)induced by the addition of 100 μM concentration of nevadensin as an in vitro model.Subsequently,the best modeled high-fat formulation was screened by constructing a fatty liver syndrome model in yellow-finned broiler chickens with three different high-fat diet formulations.Then,the effect of nevadensin was verified at the in vivo level using yellowfinned broiler chickens induced by high-fat diet as an animal model,which were divided into four groups:(1)normal control group(NC);(2)high-fat group(HFD);(3)low-dose treatment group(HFD+L): 400 mg/kg nevadensin was added to high-fat diet;(4)high-dose treatment group(HFD+H): high-fat diet800 mg/kg of nevadensin was added to the high-fat diet.Finally,the network pharmacology and molecular docking were used to find the potential targets of nevadensin in regulating lipid metabolism in broiler chickensThe results of cellular experiments showed that nevadensin could reduce lipid accumulation by decreasing oleic and palmitic acid-induced intracellular triglyceride(TG)levels in hepatocytes;improve antioxidant capacity by increasing glutathione(GSH)content and catalase(CAT)activity;promote lipolysis by increasing lipase(LPS)activity;and enhance energy metabolism by increasing the number of mitochondria and ATP content.The results of broiler experiments showed that nevadensin could reduce TG and total cholesterol(TC)levels in the liver of broiler chickens induced by high-fat diets,reduce body lipid deposition,lower serum lipid levels,alleviate liver injury and regulate the expression of genes related to lipid metabolism,thus improving the disorder of liver lipid metabolism in yellow-finned broiler chickens induced by high-fat diets and improving slaughter performance.Finally,it was hypothesized through network pharmacology and molecular docking that glycogen synthase kinase(GSK3β)might be the target of nevadensin in regulating lipid metabolism.In conclusion,nevadensin was able to alleviate high-fat-induced fat deposition and regulate hepatic lipid metabolism in broiler chickens.Nevadensin is widely available and inexpensive,and therefore has potential for further development and application.The results of this study provide a new idea for the prevention and treatment of fatty liver syndrome and related lipid metabolic diseases in chickens.