| Taxus × media(Taxus × media ’Hatfieldii’)are the largest cultivated,economically valuable and medically valuable varieties.The breed has been widely propagated since the introduction of domestication in our country in 1996.Today,there are obvious differences between the characteristics of different crops and the content of effective pharmaceutical ingredients,and enterprises lack scientific methods for identifying differences in characteristics,and there are no effective means for the material to identify the source and the origin of crops.To address the above questions,this study used the first-year and last-year-old branches of the15 year-old Taxus × media plantation in Sichuan Province as experimental materials,used methods such as plant morphology,pharmaceutical compositionology,molecular characteristics,and carries out research on the main characteristics and differences in DNA barcodes of different plantations of the Taxus × media,provides a preliminary basis for the evaluation of the quality standards of the Taxus × media,and establishes a theoretical foundation for the identification of base sources of plantation materials and the method of traceability of plantations.The study results are as follows:(1)There were significant differences in the main traits of Taxus × media materials in different planting areas.1-year-old phenotypic quality attributes showed the greatest color variance,and leaf front color was reasonably constant.Leaf area,length,density,shape index,breadth,and thickness were the phenotypic quantitative features that varied from high to low.The phenotypic quantitative features and quality attributes of Taxus × media exhibited considerable or highly significant changes across planting sites,along with leaf thickness.phenotypic quantitative characteristics and qualitative traits were strongly correlated.(2)The materials of Taxus × media in five planting areas can be divided into three groups with different characteristics.The first group Pidu(PD)of leaf length,leaf width and other traits was the highest,and the quality traits was the lowest.The second group of phenotypic quantitative traits composed of Xichang(XC)was in the middle,and the quality traits were the best.The third group composed of Dujiangyan(DJ),Jinkouhe(JK)and Hongya(HY)had the lowest leaf length and leaf width,and the quality traits were moderate.(3)The geographic and climatic characteristics of the planting location are directly related to the differences in attributes.The main characteristics of Taxus × media differ depending on the planting area’s altitude and yearly rainfall,which are significant geographical and environmental considerations.(4)Different planting sites of Taxus × media responded differently to four different DNA barcode types and their combinations.XC materials had the most DNA barcode sequences,JK materials were split into two groups with notable variances in molecular properties,and PD materials had a high degree of consistency in DNA barcodes molecular properties.(5)DNA barcodes are quite effective at locating the origin of Taxus × media.The five planting sites’ supplies came from the same source,and the combined barcode can help pinpoint where a plant was planted.The aforementioned findings demonstrated that there were various degrees of variations in the key characteristics of Taxus × media in various planting regions.The discrepancies in attributes were caused by a few geographic and environmental causes.Each planting area’s components have unique DNA barcode features.The traceability of the planting site and the identification of the source of Taxus × media are both impacted in certain ways by DNA barcodes. |
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