Investigation On Intestinal Microflora Diversity And Potential Pathogens Of Arborophila Rufipectus

The Arborophila rufipectus is a wild bird endemic to China,which belongs to the firstclass key protected wild animals.The intestinal microbial diversity of A,rufipectus is closely related to its own health.In order to understand the intestinal microbial diversity and health status of wild A,rufipectus in the reserve,the feces of wild A,rufipectus and Lophura nycthemera were collected in summer and autumn.The diversity of bacteria,fungi and parasites in the samples was analyzed by using traditional culture methods and high-throughput sequencing techniques.The test results are as follows:1.In terms of bacteria,using 16 S rRNA high-throughput sequencing technology and bacterial isolation and identification,the fecal bacteria of A,rufipectus and L,nycthemera are analyzed.the intestinal microbial diversity and richness of A,rufipectus were higher than that of L,nycthemera.,with the highest fecal species abundance in summer.The dominant phyla were Proteobacteria,Firmicutes,Bacteroidetes and Actinobacteria.The dominant genera were Shigella,Lysinibacillus,Bacteroidales＿unclassified,Enterococcus and Clostridium,etc.Some potential pathogenic bacteria,such as Shigella,Clostridium,and Acinetobacter pseudomonas were found.Functional analysis revealed that genes related to cell growth and death were found in all three groups.Shigella was also found in isolation and identification,indicating that A,rufipectus partridges and L,nycthemera may have been infected with the bacteria.2.In terms of fungi,using ITS rRNA high-throughput sequencing technology and fungi isolation and identification,the fecal fungi of A,rufipectus and L,nycthemera are analyzed there was no difference in intestinal microbial diversity and abundance between A,rufipectus and L,nycthemera.The dominant phyla were Ascomycota,Basidiomycota,Zygomycota,the dominant genera include Ascomycota＿unclassified,Fungi＿unclassified,Davidiella,Didymella,Pleosporales＿unclassified,Wickerhamomyces,and Helotiales＿unclassified,potentially pathogenic fungi such as Cryptococcus,Trichosporidium,and Candida were found.3.In terms of parasites,the faeces of A,rufipectus and L,nycthemera.were analyzed by18 SrRNA high-throughput sequencing technique.there was no significant difference between species and time.At the phylum level,Acanthocephala,Apicomplexa,Nemertea and Platyhelminthes were found.At the generic level,Plagiorhynchus,Eimeriidae＿noname,Pyramidoid,Conoidasida＿noname,Cryptosporidiidae＿noname,Hymenolepis,Nematoda＿noname,Eurytrema,Sarcocystis,Plasmodium,Strongy loides Heterakis,Toxoplasma,et al.Plagiorhynchus and Eimeriidae＿noname account for the largest proportion in genus level.This experiment is the first study on intestinal microbial diversity of A,rufipectus in China,which has very important practical significance for intestinal health,disease monitoring,early warning,prevention,and control of A,rufipectus.