Expression Of The Related-ECM Genes In Rabbit Articular Cartilage Cells After Injury

Objective:To explore the mRNA expression levels of matrix metalloproteinase-2,-3 and-9(MMP-2,-3 and-9),matrix metalloproteinase inhibitor-3(TIMP-3)and type II collagen genes in rabbit articular cartilage cells at different time-points after scratching.Methods:The articular Cartilage tissue was isolated from the articular surface of the knee joint in Adult New Zealand rabbits,and cultured primary.The 1 6-well culture plate without injury was used as normal control,the other 3 6-well culture plates cells were"#" scratched using a sterile 10μl tip,the interval space was 0.5cm,and the strength and speed of scratch were consistent.The scratching cells were harvested at 1,3 and 7 days of injury.The mRNA expressions of matrix metalloproteinases 2,3 and 9,matrix metalloproteinase inhibitors-3 and type Ⅱ collagen genes were detected using the real-time PCR.Results:The cartilage was successfully separated,the scratching injury cell model was established in primary culture cells.The mRNA expression levels of MMP-2 gene of cells at 1,3 and 7 days after injury were higher than that in normal cells,and the 7th day was the highest(P<0.05).The mRNA expression levels of MMP-3 gene of cells at 1,3 and 7 days after injury were lower than that in normal cells,and the 7th day was the lowest(P<0.05).The mRNA expression levels of MMP-9 gene of cells at 1,3 and 7 days after injury were higher than that in normal cells,and the third day was the lowest,then which began to decrease(P<0.05).The mRNA expression levels of TIMP-3 gene of cells at 1,3 and 7 days after injury were lower than that in normal cells,and the 7th day was the lowest(P<0.05).The mRNA expression levels of Col-II gene of cells at 1,3 and 7 days after injury were higher than that in normal cells,and the first day was the highest(P<0.05).Conclusions:The expression patterns of the related-ECM genes of cells at different time-points after injury is different,which can provide the basis in treating the articular cartilage injury by regulating the extracellular matrix gene expression and selecting time-point.