| Objective:To investigate the effect of glutamine(Gln)on hyperoxic lung injury in neonatal rats by Pink1-Parkin signal pathwayMethods:Ninety healthy neonatal Wistar rats were selected to prepare a model of glutamine drug intervention in hyperoxic lung injury.Random groups:air control group:(FiO2:21%,n=30);Hyperoxic group:(FiO2>85%,n=30);Drug treatment group(FiO2>85%+glutamine,n=30).From first day,the drug treatment group received intraperitoneal injection of Glutamine(Gln)at the same time0.75g/kg/d,and the control group was injected with 0.9%normal saline(NS)at the same time and at the same position as the hyperoxic group,a total of 14d.This experiment,the body weight changes of neonatal rats were measured at 3d、7d and 14d from the first day of hyperoxic model experiment.The lung tissue moisture content(W/D)of neonatal rats was measured on 3d、7d and 14d respectively;The pathological changes of lung tissues were observed by paraffin embedding and fixation with HE staining;adopt ELISA to measure IL-1β、IL-6 and TNF-αin each group’s content of the lung tissue;with Western blot method measurement of protein in the lung tissue PINK1,Parkin,Bcl2,the expression of Bax protein levels.Results:1.The weight changes of neonatal rats:compared with the control group,the weight of neonatal rats in the Hyperoxic group decreased significantly on 3d、7d and 14d(P<0.05);Compared with the hyperoxic group,the weight of neonatal rats in the drug treatment group increased significantly(P<0.05).2.Determination of lung moisture content:under the condition of high oxygen content,the lung tissue moisture content(W/D)of neonatal rats in the hyperoxic group on 3d,7d and 14d was significantly higher than that in the air control group(P<0.05).Compared with the hyperoxic group,the lung tissue moisture content(W/D)was significantly reduced in the drug treatment group(hyperoxia+Gln)and the hyperoxic group(P<0.05).3.Pathological changes in lung tissue:compared with the control group,the pulmonary alveolar structure in the hyperoxic group was more damaged,while the pulmonary alveolar structure in the control group was relatively intact.Compared with the hyperoxic group,the degree of lung tissue destruction in the drug treatment group was significantly reduced compared with that in the model group.4.Lung mediators were measured by ELISA:The contents of Il-1β,IL-6 and TNF-αon3d、7d and 14d in the hyperoxic group were significantly higher than those in the control group(P<0.05).The content in the drug treatment group was lower than that in the hyperoxic model group,which was statistically significant(P<0.05).5.Western blot method:compared with the control group,the expressions of PINK1,Parkin and Bax proteins were significantly increased on 3d,7d and 14d in the hyperoxy model group(P<0.05),and the expressions of Bcl-2 proteins were significantly decreased,with statistical significance(P<0.05);The expression of PINK1,Parkin and Bax proteins in the drug treatment group was significantly lower than that in the model group,with statistical significance(P<0.05).Conclusions:1.Glutamine(Gln)can relieve the edema of neonatal rat lung tissue and the infiltration of inflammatory factors caused by hyperoxia.2.The protective mechanism of Glutamine(Gln)on neonatal rats in hyperoxic lung injury is related to the regulation of the expression of PINK1,Parkin,Bax and Bcl-2 proteins through mitochondria. |
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