| Cancer is still one of the diseases that seriously threaten our life.Recently,the rapid development of nanotechnology has provided new ideas for cancer diagnosis and therapy.Among them,metal-organic framework materials(MOF)as novel emerging hybrid materials,have many advantages,such as large specific surface area,high porosity,and adjustable structure and function,etc.It has great potential to become a new powerful generation of tumor theranostics nanoplatform.It is essential to solving the targeting problem for the realization of tumor diagnosis and therapy function.The biomimetic technology of cancer cell membrane is based on the principle of bionics,which has good biocompatibility to achieve tumor targeting ability and is quite different from those exogenous post-synthesis strategies.Based on this,we designed and constructed a near-infrared fluorescent probe and nanoscale tumor theranostics platform based on the cancer cell membrane-coated porphyrin MOF.Nanoparticles that inherit the advantages of cancer cell membranes can be enriched in tumor tissues and achieve enhanced tumor theranostics efficacy.1.Biomimetic porphyrinic metal-organic framework nanoparticles as a novel near-infrared fluorescent probe for tumor imaging and detection.In this work,the encapsulation method was utilized to load the near-infrared fluorescent dye indocyanine green(ICG)into the porphyrinic MOF(PCN-224)to obtain the near-infrared fluorescent probe(ICG@PCN-224),followed by cell membrane coating to obtain biomimetic near-infrared fluorescent probe ICG@PCN-224@mem.The obtained near-infrared fluorescent probe has suitable size distribution,uniform particle size,and good dispersion.In vivo experiment,after being intravenously injected into the tumor-bearing mice,the probe can accumulate in the tumor tissue to achieve enhanced near-infrared fluorescence imaging.Besides,the blood circulation time of the fluorescent dye ICG is remarkably prolonged,which is more conducive for nanoprobes to achieve tumor targeting.These results prove that the prepared near-infrared fluorescent probe with tumor targeting and specific recognition ability is an excellent tumor imaging choice.2.Cancer membrane-coated porphyrinic metal-organic framework nanoparticles as superior drug nanocarriers for imaging-guided synergistic cancer therapy.Recently photodynamic therapy is an emerging cancer treatment procedure.However,it has limited therapeutic effects on deep tumors and distant tumors due to the dependence on lasers.Therefore,it is necessary to develop more effective photodynamic systems and more treatment methods.In this work,we constructed a hollow porphyrinic metal-organic framework material(H-PMOF),which not only has a stronger photodynamic therapeutic effect than the non-hollow porphyrin MOF but also provides a highly efficient drug loading content due to the hollow structure.The co-loading content of chemotherapeutic drug doxorubicin and photothermal reagent ICG in H-PMOF was 635%.Besides,after using the natural homologous targeting characteristics of cancer cell membranes to realize camouflage,the obtained nanoplatform showed good biocompatibility in vitro and in vivo studies and could achieve excellent tumor enrichment in tumor-bearing mice.The three-modal(photodynamic\photothermal\chemotherapy)synergistic anti-tumor strategy demonstrated excellent primary tumor ablation and distal tumor suppression at ultralow doses.This highly efficient tumor theranostic nanoplatform which based on cancer cell membrane biomimetic technology has great potential for future clinical applications. |
