Expression And Correlation Of Short-chain Fatty Acid Receptors In Inflammatory Bowel Disease

Background and aims:FFAR2 and FFAR3 are activated by short-chain fatty acids and regulate a variety of inflammatory and immune responses.The role of short-chain fatty acid receptors in IBD has been extensively studied in animal experiments.This study aims to investigate the expression levels of FFAR2 and FFAR3 in intestinal mucosal tissues of patients with inflammatory bowel disease and its correlation with disease severity,so as to further explore the pathogenesis of IBD and provide a potential target for the treatment of IBD.Methods:In this study,the data set GSE94648(22 healthy patients and 75 IBD patients)was downloaded from GEO database,and 435 genes up-regulated in IBD were screened by differential gene analysis.A group of genes with similar expression pattern that had the highest correlation with IBD were found by WGCNA,and the highly expressed hub genes FFAR2 and FFAR3 were further screened out.A total of 41 patients with inflammatory bowel disease(22 CD patients and 19 UC patients)admitted to our hospital from June 2020 to January 2021 were collected.Patients were grouped according to endoscopic score and disease activity score,and their normal tissue was used as control.Immunohistochemical staining,western blot and fluorescence quantitative PCR were used to detect the expression level of FFAR2 and FFAR3,and the correlation between its expression and the severity of disease was analyzed.Results:Immunohistochemical staining results showed that the positive expressions of FFAR2 and FFAR3 proteins in the intestinal mucosal tissues of patients with CD and UC were significantly higher than those in the normal control group,with brown-yellow staining as the dominant color.Compared with normal control group,the protein and mRNA expression levels of FFAR2 and FFAR3 in intestinal mucosa of patients in CD group and UC group were increased(P<0.05),and the expression level increased with the increase of disease severity.Conclusion:The expression of short-chain fatty acid receptor(FFAR2 FFAR3)in intestinal mucosal tissues of patients with IBD is increased,and it is closely related to the severity of the disease.