Study On The Chemical Constituents Of The Seeds Of Tripterygium Wilfordii Hook.F. And Their Biological Activities

As one of the countries with the richest plant diversity,the number of China’s plant species ranks third in the world,which make it convenient for us to systematically study the chemical constituents of some traditional and folk medicinal plants in our country.On the one hand,it provides lead compounds for the discovery of innovative drugs;on the other hand,it is of great importance to provide a scientific basis for the comprehensive development and utilization of medicinal plants in China.In this thesis,the seeds of the medicinal plant Tripterygium wilfordii were taken as the research object.We studied its chemical constituents and biological activities.A total of 50 dihydro-β-agarofuran-type sesquiterpenoids(1～50),including 24 new compounds,were identified.The absolute configurations of 14 compounds were determined by CD exciton chirality methods,single-crystal X-ray diffraction analysis and ECD calculation methods.Bioassay showed that compounds 31,35 and 47 significantly inhibited Con A-induced T cell proliferation and LPS-induced B cell proliferation,compounds 36 and 39 were found to significantly inhibit Con A-induced T cell proliferation,and compound 40 exhibited significant inhibitory effect on LPS-induced B cell proliferation.In addition,among the 50 compounds isolated,most of the compounds could significantly inhibit the differentiation of bone marrow osteoclast precursors into osteoclasts induced by RANKL at the concentration of 10μM,and the inhibition rate of 36 compounds was more than 95%.According to the above results,the chemical constituents of the seeds of Tripterygium wilfordii was improved,the bioactive species of dihydro-β-agarofuran-type sesquiterpenoids were enriched,and the material basis for other bioactivity testing was provided.