Correlation Between Vaginal Microecology And Immune Status And High-risk HPV Infection In Patients With Cervical Lesions

Objective: Through research and analysis of vaginal microecology and detection of the concentrations of IL-2,IL-10 and CD4 in vaginal lavage fluid,To explore the correlation between vaginal microecology and vaginal local immune status changes with cervical lesions and cervical high-risk HPV infection.It aims to provide new ideas and new directions for early prevention and intervention of cervical lesions.Methods: 1.According to the inclusion and exclusion criteria formulated in the study,40 women diagnosed with LSIL,HSIL and CSCC were included as the observation group,and 120 women with negative TCT or TS and cervical HPV double screening results in our hospital during the same period were selected.As the control group,the state of vaginal microecology in the two groups was observed,and the differences between the two groups were compared and analyzed.2.Among the 240 subjects,110 were positive for cervical high-risk HPV and 130 were negative for HPV,to compare the relationship between vaginal microecology and cervical high-risk HPV infection.3.Randomly select 4 groups(including 20 cases in the control group,20 cases in the LSIL group,20 cases in the HSIL group,and 20 cases in the CSCC group),a total of 80 research objects in the vaginal lavage fluid,and detect IL-2,IL-10 and CD4 respectively.Concentrations were compared and analyzed the differences between vaginal local immune status and cervical lesions of different grades and cervical high-risk HPV infection.SPSS26.0 statistical software was used for statistical analysis,in which enumeration data were expressed as rate(%),and analyzed by chi-square test or Fisher’s exact probability method;measurement data in line with normal distribution were expressed as mean±standard deviation(X±S)Expressed,using analysis of variance for comparison,comparison between groups using post-hoc test;non-normal distribution expressed as median(M)and quartile(IQR),using nonparametric test for statistical analysis,two groups of independent samples Mann-Whitney test was used for comparison,and Kruskal-Wallis test was used for multiple independent samples.P<0.05 was considered statistically significant.Results: 1.In the vaginal microecology of the observation group and the control group,the positive rate of BV,PH≥4.6,the positive rate of sialidase,the positive rate of proline aminopeptidase and the positive rate of coagulase were all different in the observation group.increased,and the difference between the two groups was statistically significant(P<0.05).2.In the vaginal microecology among the three groups of LSIL,HSIL and CSCC,the PH≥4.6 showed an increasing trend with the aggravation of cervical lesions,and the difference between the groups was statistically significant(P<0.05);BV positive rate,coagulation The positive rate of enzyme and acetylglucosaminidase decreased first and then increased with the aggravation of cervical lesions,and increased significantly in CSCC group,and the difference between groups was statistically significant(P<0.05).3.The positive rate of VVC in the vaginal microecological analysis in the cervical high-risk HPV positive group was lower than that in the HPV negative group,and the difference between the two groups was statistically significant(P<0.05).PH≥4.6,leukocyte esterase positive rate,sialidase positive rate,proline aminopeptidase positive rate and coagulase positive rate all increased in cervical high-risk HPV positive group,compared with HPV negative group The difference was statistically significant(P<0.05).4.In the vaginal lavage fluid of the control group,LSIL group,HSIL group and CSCC group,The medians of IL-2 concentrations were: 6.52 pg/ml,8.74 pg/ml,9.75pg/ml,6.99 pg/ml,the median first increased and then decreased with the aggravation of cervical lesions,and there was no statistical difference between the four groups significance(P>0.05);the medians of IL-10 concentrations were:11.66 pg/ml,14.22 pg/ml,40.19 pg/ml,344.09 pg/ml,and the medians increased with the aggravation of cervical lesions.There were significant differences between groups(P<0.05);the medians of CD4 concentrations were: 0.67 ng/ml,0.68 ng/ml,1.13 ng/ml,2.49 ng/ml,and the medians increased with the aggravation of cervical lesions.There was an increasing trend,and the difference between the four groups was statistically significant(P<0.05).5.In the vaginal lavage fluids of the HPV-negative and cervical high-risk HPV-positive groups,the median IL-2 concentrations were 9.56 pg/ml and 8.67pg/ml,respectively,and there was no significant difference between the two groups(P>0.05).);the median concentrations of IL-10 were: 14.81 pg/ml,49.10pg/ml,and the difference between the two groups was statistically significant(P<0.05);the median concentrations of CD4 were: 0.76 ng/ml,1.33 ng/ml,there was no significant difference between the two groups(P>0.05)Conclusion: 1.Vaginal microecological imbalance is related to cervical lesions;BV positive,PH ≥ 4.6,sialidase positive,proline aminopeptidase positive and coagulase positive may be related to the occurrence of cervical lesions;among which BV positive,PH ≥ 4.6,sialidase positive and acetylglucosaminidase positive may be related to promoting the occurrence and development of cervical cancer;2.Vaginal microecological imbalance is related to cervical high-risk HPV infection;BV positive,PH≥4.6,white blood cells Esterase positive,sialidase positive,proline aminopeptidase positive and coagulase positive may be related to high-risk HPV infection;3.Vaginal local immune disorders are related to cervical lesions and cervical high-risk HPV infection;IL-10 concentration The increase may promote the occurrence of cervical lesions and cervical high-risk HPV infection.